1993 Fiscal Year Final Research Report Summary
Signal Transduction Mechanisms of Nerve and Immune Cells.
| Project/Area Number |
03454130
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Shimane Medical University |
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Principal Investigator |
SAWADA Masashi Shimane Med. Univ., Associate Prof., 医学部, 助教授 (20019558)
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| Co-Investigator(Kenkyū-buntansha) |
ICHINOSE Mitsuyuki Shimane Med. Univ., Instructor, 医学部, 助手 (90127506)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Mouse peritoneal macrophage / Aplysia neuron / Voltage-clamp / Patch-clamp / Ionic mechanisms / Phagocytosis / G protein / Homeostasis |
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| Research Abstract |
Recent studies have shown that there may exist a bidirectional communication between the nervous ystem and the immune system. The concept that the brain and the immune system communicate with each other is supported by the presence of signal molecules and the receptors common to both systems. For example the immune system may signal the brain through chemical messengers, like cytokines derived from immunological cells. On the other hand, the brain may module the immune functions by the neuroendocrine communication and neural connections of autonomic nervous sysmtem. 1)Responses to adrenaline in mouse peritoneal macrophages were investigated with perforated and cell-attached patch-clamp recording. It is concluded that adrenaline activates a Ca^<2+>-dependent K^+ conductance resulted from stimulation of alpha_<1A>-adrenoceptor and InsP_3 formation via a G protein. 2) The ionic mechanism of the effects of extracellularly ejected interleukin-1 (IL-1) and tumor necrosis factor (TNF) on membrance of identified neurons of Aplysia was investigated with voltageclamp and micropressure-ejection techniques. The results demonstrated that IL-1 can induce a slow outward current associated with a decrease in Na^+ conductance, and TNF can induce a slow inward current via a decrease in K^+ conductance, respectively.
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