1993 Fiscal Year Final Research Report Summary
The expression of mouse TL antigens and its relationship to gammadelta T cells
| Project/Area Number |
03454198
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
OBATA Yuichi Aichi Cancer Center, Lab.of Immunology, Section Head, 免疫学部, 室長 (30177290)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Osamu Aichi Cancer Center, Lab.of Experimental Pathology, Senior Researcher, 病理学・第二部, 主任研究員 (00142167)
TAKAHASHI Toshitada Aichi Cancer Center, Vice Director, 副所長 (00124529)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Mouse TL antigen / MHC class I antigen / Transcription factor / Thymic T cell / Intestinal epithelium / Cytotoxic T cell / gammadelta T cells / Antigen presentation |
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| Research Abstract |
Mouse TL antigens belong to a family of the major histocompatibility complex (MHC) class I antigens. However, the expression is distinct from other MHC class I antigens, being restricted to normal thymocytes, epithelial cells of the intestine and T cell lymphomas. The aims of the present study were to elucidate the function of TL as well as the structural basis for TL expression. The study was aimed also to delineate the function and maturation steps involved in the differentiation of gammadelta T cells. The results obtained through the study are summarized as follows.
1. Regulation of TL expression : (1) The tissue-specific promoter of TL genes is present in an 80-bp region of the 5' flanking sequence. (2) The nuclear extract of normal thymocytes contains the factor(s) which bind to a DNA fragment containing the promoter region.
2. The function of TL antigens in thymus : The cytotoxic T cell (CTL) clones against TL antigens were established from atransgenic mouse which expresses TL in t
… More
he thymus and from a C3H mouse which does not. Two of eight CTL clones from transgenic mouse were gammadelta T cells, while all 15 clones from C3H were alphabeta T cells. Thus, the results suggested that the expression of TL in the thymus leads to the selection of T cells belonging to the gammadelta lineage.
3. The function gammadelta T cells. Two gammadelta T cells clones with cytotoxic activity against TL antigens were established as mentioned above. The gammadelta CTL clones do not require an antigenic presentation by H-2 molecules. This indicated that T cell receptors of these cells recognize TL directly, and implied further the function of TL as an antigenic presenting molecule.
4. TL expression in the intestinal epithelium. TL antigens are expressed in the epithelial cells of the small and large intestines of all mice so far tested, including those which do not TL in the thymus. TL antigens are present on the cell surface and cytoplasm of the epithelial cells of villi. This had led to the hypothesis that the major function of TL is to present antigens to neighboring T cells in the gut. Less
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Research Products
(33 results)
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[Publications] Morita, A., Takahashi, T., Stockert, E., Nakayama, E., Tsuji, T., Matsudaira, Y., Old, L.J., and Obata, Y.: "TL-antigen as a transplantation antigen recognized by TL-restricted cytotosic T cells." J.Exp.Med.179. 777-784 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Obata, Y., Satta, Y., Moriwaki, K., Shiroishi, T., Hasegawa, H., Takahashi, T., and Takahata, N.: "The structure, function and evolution of mouse TL genes, nonclassical class I genes of the major histocompatibility complex." Proc.Natl.Acad.Sci, U.S.A.(in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Watanabe, H., Ohtsuka, K., Obata, Y., Iiai, T., Kimura, M., Takahashi, T., Hirokawa, K., Utsuyama, M., and Abo, T.: "Generalized expansion of extrathymic T cells in various immune organs of TL-transgenic mice." Biomed.Res.14. 273-288 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshikawa, K., Seto, M., Ueda, R., Obata, Y., Fukatsu, H., Segawa, A., and Takahashi, T.: "Isolation and characterization of mouse CD7 cDNA." Immunogenetics. 37. 114-119 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sekido, Y., Takahashi, T., Makela, Y.P., Obata, Y., Ueda, R., Hida, T., Hibi, K., Shimokata, K., Alitaro, K., and Takahashi, T.: "Complex intrachromosomal rearrangement in the process of amplification of the L-myc gene in small cell lung cancer." Mol.Cell.Biol.12. 1745-1754 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takagi, N., Nakamura, S., Ueda, R., Osada, H., Obata, Y., Kitoh, K., Suchi, T., and Takahashi, T.: "Phenotype and genotypic study of node-based low grade peripheral T cell lymphoma : angioimmunoblastic" Cancer.69. 2571-2582 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Obata, Y., Taguchi, O., Matsudaira, Y., Hasegawa, H., Hamasima, N., and Takahashi, T.: "Abnormal thymic development, impaired immune function and gammadelta T cell lymphomas in a TL transgenic mouse strain." J.Exp.Med.174. 351-362 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Mieno, M., Suto, R., Obata, Y., Udono, H., Takahashi, T., Shiku, H., and Nakayama, E.: "CD4^-CD8^- T cell receptor alphabeta T cells : generation of an invitro major histocompatibility complex class I specific cytotoxic T lymphocyte response and allogeneic tumor rejection." J.Exp.Med.174. 193-201 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshikawa, K., Seto, M., Ueda, R., Obata, Y., Notake, K., Yokochi, T., and Takahashi, T.: "Molecular cloning of the gene coding for the human T cell differentiation antigen CD7." Immunogenetics. 33. 352-360 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sekido, Y., Obata, Y., Ueda, R., Hida, T., Suyama, M., Shimokata, K., Ariyoshi, Y., and Takahashi, T.: "Preferential expression of c-kit protooncogene transcripts in small cell lung cancer" Cancer Res.51. 2416-2419 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kawai, M., Obata, Y., Takahashi, T., Uenaka, A., Monden, M., Mori, T., Shiku, H., and Nakayama, E.: "Differential involvement of CD4^+ cells in mediating skin graft rejection against different amounts of transgenic H-2K^b antigen." J.Exp.Med.173. 261-264 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsuda, R., Takahashi, T., Nakamura, S., Sekido, Y., Nishida, K., Seto, M., Seito, Y., Sugiura, T., Ariyoshi, Y., Takahashi, T., and Ueda, R.: "Expression of the c-kit protein in human solid tumors and in corresponding fetal and adult normal tissues." Amer.J.Pathol.142. 339-346 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Emi, N., Kitaori, K., Seto, M., Ueda, R., Saito, H., Takahashi, T.: "Isolation of a novel cDNA clone showing a marked similarity to ME491/CD63 superfamily." Immunogenetics. 37. 193-198 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Seo, N., Okazaki, T., Nakanishi-Ito, C., Tanino, T., Matsudaira, Y., Takahashi, T., Egawa, K.: "Expression Qa-2^k phenotype encoded by the Q5^k gene on the surface of tumor cells derived from H-2^k mice." J.Exp.Med.175. 647-654 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Taguchi, O., Kontani, K., Ikeda, H., Kezuka, T., Takeuchi, M., Takahashi, T., andTakahasi, T.: "Tissue-specific suppresser T cells involved in self tolerance are activated extrathymically by self antigens." Immunology. (in press). (1994)
Description
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[Publications] Ichikawa, T., Taguchi, O., Takahashi, T., Ikeda, H., Takeuchi, M., Tanaka, T., Usui, M.and Nishizuka, Y.: "Spontaneous development of autoimmune uneoretinitis in nude mice following reconstitution with embryonic rat thymus." Clin.Exp.Immunol.85. 112-117 (1991)
Description
「研究成果報告書概要(欧文)」より
