1992 Fiscal Year Final Research Report Summary
Developmental Research on Preservation of Liver Graft by Improving the Hepatic Microcirculation
| Project/Area Number |
03454231
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
SATO Nobuhiro Deaprtment of GE ,Juntendo Univ. Sch. Med. Prof., 医学部, 教授 (90028358)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEI Yoshiyuki 1st Dept of Med, Osaka Univ Med Sch ,Physician, 医学部・附属病院, 医員
MIYAZAI Akihisa Dept of GE Juntendo Univ Sch of Med Assistant, 医学部, 助手 (20200149)
YOKOI Yukio Dept of GE Juntendo Univ Sch of Med Lecturer, 医学部, 講師 (20150633)
WATANABE Sumio Dept of GE Juntendo Univ Sch of Med, Lecturer, 医学部, 講師 (20138225)
KITAMI Noriyuki Dept of GE Juntendo Univ Sch of Med ,Assoc Prof, 医学部, 助教授 (90053256)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Liver Transplantation / Microcirculation / Preservation of Liver Graft / Cytoskeleton / Kupffer cells / Oxygenation / Ischeima-referfusion Injury / Ito cells |
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| Research Abstract |
In this project, we investigated (1)the role of hepatic microcirculation on the re-perfusion injury and (2)the preservation condition for the graft in liver transplantation.
The mechanisms to control sinusoidal blood flow were investigated using cultured Ito cells and Kupffer cells which exist in sinusoid. Immunohistochemical staining revealed that contractile proteins such as actin, myosin and desmin were richly distributed in Ito cells. Addition of vasoactive substance, endothelin-1, caused the contraction of Ito cells and this contractile activity was thought to contribute on sinusoidal contraction and control of blood flow. Kupffer cells showed active phagocytic activity with the actomyosin system and Kupffer cells with ingested foreign substances might block the sinusoidal blood flow. The predictive factors for the re-perfusion injury was investigated and revealed that the oxygenation of the surface of the liver was a best available for prediction of the prognosis of liver transplantation.
Although, the UW solution is standard for the liver preservation, we developed a new solution called "Carolina Rinse solution" for the better preservation. The clinical trial to assess the benefit of this solution for the liver transplantation has begun in Mayo Clinic in USA.
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