1992 Fiscal Year Final Research Report Summary
Basic study to establish artificial lung with metabolic functions
| Project/Area Number |
03454234
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KITAMURA Satoshi Jichi Medical School, Pulmonary Medicine, Professor, 医学部, 教授 (60010427)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Tatsuya Jichi Medical School, Pulmonary Medicine, Assistant Professor, 医学部, 講師 (40225719)
OHNO Syoji Jichi Medical School, Pulmonary Medicine, Assistant Professor, 医学部, 講師
KOBAYASHI Jun Jichi Medical School, Pulmonary Medicine, Assistant Professor, 医学部, 講師 (90195785)
SUGAMA Yasuo Jichi Medical School, Pulmonary Medicine, Assistant Professor, 医学部, 講師 (20187636)
SUGIYAMA Yukihiko Jichi Medical School, Pulmonary Medicine, Associate Professor, 医学部, 助教授 (70183415)
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| Project Period (FY) |
1992
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| Keywords | Artifioal lnng / Arachidonic acid / Alveolar macrophage / Endothelial cell / Leukotriene / Prostaglandin / Thromboxane / Endotheline |
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| Research Abstract |
Basic experiments were performed about alveolar macrophages and endothelial cells for these two years.
Arachidonic acid metabolism of alveolar macrophages was analyzed by guinea pig alveolar macrophages. Macrophages were obtained by bronchoalveolar lavage, and incubated with arachidonic acid and A23187 for 5 minutes. Metabolites of supernatant were analyzed by reverse phase high-performance liquid chromatography. Leukotriene B_4, 5-HETE, and HHT were detected and the production of thromboxane A_2 was also speculated. Because these metabolites have very high chemotactic and bronchoconstrictive activities, it may be suggested that the metabiolites of arachidonic acid are very important to maintain the homeostasis of pulmonary function.
On the other hand, metabolic functions of cultured endothelial cells were analyzed under the influence of leukotrienes, nicotine, and elastase. Leukotriene B_4 and D_4 stimulated the release of prostaglandin I_2 and thromboxane B_2 from endothelial cells. Nicotine inhibited the release of prostaglandin I_2, thromboxane B_2 and endotheline from endothelial cells in higher concentration. Elastase inhibited the permeability of cultured monolayer of endothelial cells. These results demonstrate that endothelial cells can regulate not only tonus of smooth muscle of airway and blood vessels but also chemotactic activities through the release of various chemical mediators.
These basic data will provide us some basic information to establish an artificial lung with metabolic function.
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