1993 Fiscal Year Final Research Report Summary
Cell biological analysis on hair growth mechanism
| Project/Area Number |
03454276
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
OGAWA Hideoki Juntendo University, Dept.of Dermatology, Professor, 医学部, 教授 (30053151)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Ryusuke Juntendo University, Dept.of Dermatology, M.D., 医学部, 助手 (70175214)
TAKAMORI Kenji Juntendo University, Dept.of Dermatology, Professor, 医学部, 教授 (40053144)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Hair growth / Hair germinative cells / Hair dermal papillar cells / Organ culture / Cell culture / Hepatocyte growth factor(HGF) |
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| Research Abstract |
We analyzed the effects of various cytokines on hair growth using an organ culture system with serum-free medium.
I.Effects of cytokines :
a) a-FGF, b-FGF, TGF-alpha and IL-6 had no influence on hair growth.
b) k-FGF, EGF, TGF-beta, IGF-1, IL-alpha and TFNF-alpha inhibited hair growth and DNA synthesis of hair follicles.
c) HGF significantly stimulated both hair growth and DNA synthesis of hair follicles. The effects of HGF were blocked by an HGF-specific neutralizing antiserum.
II.Expression of HGF on hair dermal papilla cells :
a) HGF immunoreactivities in conditioned media of dermal papilla cells were detected by EIA.
b) Using the RT-PCR method, HGF mRNA expression of dermal papilla cells was detected.
These results indicate that HGF can promote hair growth and may have clinical utility in this regard.
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