1993 Fiscal Year Final Research Report Summary
Electromicroscopic and histochemical analysis in metabolism of the fat emulsion synthesized with new phospholip
| Project/Area Number |
03454308
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Wakayama Medical College |
|---|
Principal Investigator |
TANIGUCHI Katsutoshi Wakayama Medical College, Associate Proffessor, 消化器外科, 助教授 (90106568)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MANIWA Yoshio Wakayama Medical College, Associate Proffessor, 消化器外科, 講師 (50229598)
NAGAI Yugo Wakayama Medical College, Associate Proffessor, 消化器外科, 講師 (00172495)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Keywords | phospholipids / fat emulsion / HPLC / phosphatidylglycerol |
|---|
| Research Abstract |
We produced a stable fat emulsion with a amall particle diameter by new liquid crystal emulsification method using phosphatidylglycerol, phosphatidylpolyethyleneglycol, and phosphatidyglucose, which are anionic surfactants, as emulsifiers. Electrom microscopy of the metabolism of the new fat emulsion in the liver by fat particle fixation method showed rapid uptake of fat particles and metabolism in liver cells compared with the conventional emulsion. In addition, the phospholipids and neutral fat in this emulsion were separately labeled with trltium and their pharmscokinetics was evaluated. Not only the phospholipids but also neutral fat was rapidly incorporated into the liver and metabolized. The phospholipids as emulsifiers markedly affected the organ directivity of neutral fat. For separation and analysis of phospholipids with different properties according to the class, a HPLC system using the post column method was developed. This system allowed separation and quantification of 6 glyceropholipids such as phosphatidylglycerol. The disappearance rate of these emulsifiers from the blood was higher than that of conventional yolk lecithin, suggesting only slight accumulation in the body.
In terms of metabolism of fat emulsion, it os improtant to take into account not only the metabolism of neutral fat but also that of phospholipids. These phospholipids are rapidly metabolized in the body and may be useful as fat emulsifiers.
|
