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1992 Fiscal Year Final Research Report Summary

PREVENTION OF ISCHEMIA-REPERFUSION INJURY AND ESTABLISHMENT OF THE METHODS FOR CARDIAC PRESERVATION AND REPERFUSION

Research Project

Project/Area Number 03454334
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KOHNO Hiroyuki  KYUSHU UNIV., MEDICINE,ASSISTANT PROF., 医学部, 助手 (80178227)

Co-Investigator(Kenkyū-buntansha) MASUDA Munetaka  KYUSHU UNIV., MEDICINE, ASSISTANT PROF., 医学部, 助手 (10190365)
TOMINAGA Ryuji  KYUSHU UNIV., MEDICINE, LECTURER, 医学部, 講師 (70136464)
KAWACHI Yoshito  KYUSHU UNIV., MEDICINE, LECTURER, 医学部, 講師 (80117093)
YASUI Hisataka  KYUSHU UNIV., MEDICINE, ASSOCIATE PROF., 医学部, 助教授 (20089923)
Project Period (FY) 1991 – 1992
KeywordsMYOCADIAL PROTECTION / CARDIOPLEGIA / ISCHEMIA / REPERFUSION INJURY / ACE INHIBITOR / CAPTOPRIL / ISOLATED CARDIAC MYOCYTE / INTRACELLULAR CALCIUM CONCENTRATION
Research Abstract

1. To prevent intracellular calcium overload, we investigated the hyperpolarizing cardioplegia which we had made in 1991. The hyperpolirizing cardioplegia consists of low potassium and contains adenosine and pinacidil (potassium channel opener). As it keeps cell membrane hyperpolirizing, we assume that L-type calcium channels on cardiac myocyte is kept close and that intracellular calcium overload was prevented. We compared the protective effect of the hyperpolarizing cardioplegia with that of ordinary high potassium cardioplegia in isolated rat heart model. The protective effect of hyperpolarizing cardioplegia was similar to that of the high potassium cardioplegia.
2. We investigated the effects of cardioplegia containing angiotensin converting enzyme (ACE) inhibitor on ischemia/reperfusion in rat hearts. The cardioplegia contaning 2.5mmole/L captopril showed good myocardial protection during reperfusion but not during ischemia. Another ACE inhibitor, Enalapril and Lisinopril showed no effect during neither ischemia nor reperfusion. Because only captopril has a sulfhydryl, we considered myocardial protective effect of captopril might be due to free radical scavenging rather than ACE inhibition.
3. We planed to measure intracellular calcium concentration and intracellular pH during ischemia and reperfusion in isolated cardiac myocytes. We succeeded to get enough myocytes from ventricular muscle of rat for experiments to measure both intracellular calcium concentration and pH by loading of Fura-2 and BCECF. Because we have not yet succeeded to keep isolated myocyte in ischemia, we cannot report fully about this experiment.
4. Now, we are making a new experimental model in which we measure intracellular calcium concentration and pH in isolated rat of rabbit heart at global ischemia. When we establish this model, it might be one of the best model to examine calcium homeostasis during ischemia and reperfusion.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Atsuo Mitani: "The effects of glibenclamide and nicorandil on cardiac function during ischemia and reperfusion in the isolated perfused rat heart." American Journal of Physiology. 261. H1864-H1871 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhiko Kinoshita: "Analysis of determinants of Ventricular fibrilation induced by reperfusion:Dissociation between electrical instability and myocardial damage." The Annals of Thoracic Surgery. 53. 999-1005 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuhiko Kinoshita: "Delayed time to peak serum myoglobin level as an indicator of cardiac dysfunction following open heart surgery." Chest. 99. 1572-1581 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Toshima: "Collins' solution for cols storage of the heart for transplantation must be reserved with cardioplegic solution before reperfusion." The journal of Thoracic and Cardiovaswlar Surgery. 104. 1572-1581 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Munetaka Masuda: "Effects of nucleoside transport inhibition on long-term ex vivo preservation of canine hearts." The journal of Thoracic and Cardiovaswlar Surgery. 104. 1610-1617 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鶴原 由一: "心臓手術とフリーラジカル" Journal of Active Oxygens & Free Radicals. 2. 746-756 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitani A.: "The effects of glibenclamide and nicorandil on cardiac function during ischemia and reperfusion in the isolatedperfused rat heart." Am J Physiol. 261. H1864-H1871 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita K.: "Analysis of determinants of ventricular fibrilation induced by reperfusion: Dissociation between electrical instability and myocardial damage." Ann Thorac Surg. 53. 999-1005 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita K.: "Delayed time to peak serum myoglobin level as an indicator of cardiac dysfunction following open heart surgery." Chest. 99. 1398-1402 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshima Y.: "Collins' solution for cold storage of the heart for transplantation must be reserved with cardioplegic solution before reperfusion." J Thorac Cardiovasc Surg. 104. 1572-1581 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuda M.: "Effects of nucleoside transport inhibition on long-term ex vivo preservation of canine hearts." J Thorac Cardiovasc Surg. 104. 1610-1617 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Turuhara Y.: "Free radicals in cardiac surgery." J Act Oxyg Free Rad. 2. 746-756 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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