Analysis of the genes involved in the growth of human professor

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 03454388
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: KUBOTA Yoshinobu Yokohama City Univ.School of Medicine, Associate Professor, 医学部, 助教授 (10106312)
• Co-Investigator(Kenkyū-buntansha): SHUIN Taro Yokohama City Univ.School of Medicine Assistant Professor, 医学部, 講師 (80179019) ; HOSAKA Masahiko Yokohama City Univ.School of Medicine Professor, 医学部, 教授 (30106330)
• Project Period (FY): 1991 – 1993
• Keywords: Prostate Cancer / Oncogene / Tumor suppressor gene / Growth facor / Androgen Receptor

Research Abstract

The growth mechanisms of human prostate cancer is unclear. Here, we investigated the expression and structural abnormalities of several genes which associated with cell cycle and growth factors and their receptors to understand the molecular background of the abnormal growth of human prostate cancer.
We analyzed the abnormalities of the oncogenes, tumor suppresser genes and their associated genes in the 31 tumor samples from prostate cancer patients and cell lines derived from human prostate cancers with several methods of molecular biology.
Abnormalities were found in myc, P53, RB and DNA-polymerase B and Androgen Receptor genes.
These results suggested that the abnormalities of these genes including mutations have important roles in the progression processes of human prostate carcinogenesis and also give us the hints for further studies as well as the design of gene therapy and gene diagnosis.

Research Products

• [Publications] Dobashi,Y.et al: "DNA polymerase B gene mutation in human prostate cancer" Cancer Research. 54. 2827-2829 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kubota,Y.et al: "Tumor suppressor gene PS3 mutations in human prostate cancer" The Prostate. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Dobashi,Y.et al: "Polymorphism in human DNA polymerase B gene" Human Genetics. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kubota Y.et al: "Fundamental approaches to the diagnosis and treatment of prostate cancer and BPH" Adenin Press, 213 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Dobashi, Y., Shuin, T., Tsuruga, H., Uemura, H., Torigoe, S., and Kubota, Y: "DNA polymerase beta gene mutation in human prostate cancer" Cancer Res. 54. 2827-2829 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kubota, Y., Shuin, T., Uemura, H., Fujinami, K., Torigoe, S., Dobashi, Y., Iwasaki, Y., Daneneberg, K., and Daneneberg, P.V: "Tumor suppressor gene p53 mutations in human prostate cancer" The Prostate. 27. 18-24 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Dobashi, Y., Kubota, Y., Shuin, T., Torigoe, S., and Hosaka, M: "Polymorphism in human DNA polymerase beta gene" Human Genetics. (in press). (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kubota, Y., Fujinami, K., Uemura, H., Miyamoto, H., Dobashi, Y., Shuin, T., Hosaka, M., Iwasaki, Y., and Kitamura, H: Analysis of tumor suppressor genes in human prostate cancer by RNA-SSCP method. Adenin Press, 157-168 (1994)
  • Description: 「研究成果報告書概要(欧文)」より