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1993 Fiscal Year Final Research Report Summary

Investigations on physiological functions of rabbit eye suprachoroid.

Research Project

Project/Area Number 03454413
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HIRAI Keiji  Tokyo Medical and Dental University, Medical Research Institute, Associate Professor, 難治疾患研究所, 助教授 (70156628)

Co-Investigator(Kenkyū-buntansha) FUNATA Midori  Tokyo Medical and Dental University, Faculty of Medicine, Lecturer, 医学部, 講師 (10143554)
TOKORO Takashi  Tokyo Medical and Dental University, Faculty of Medicine, Professor, 医学部, 教授 (20013865)
KATAYAMA Yoshifumi  Tokyo Medical and Dental University, Medical Research Institute, Professor, 難治疾患研究所, 教授 (20014144)
Project Period (FY) 1991 – 1993
KeywordsSuprachoroid / Fura-2 / Intracellular calcium concentration / Calcium homeostasis / Adenosine triphosphate / Uridine triphosphate / Nucleotide receptor / Inositol triphosphate
Research Abstract

The physiological function of suprachoroid was investigated. Intracellular calcium concentration of rabbit eye suprachoroid was measured by means of microfluorophotometry with a fluorescent calcium indicator, fura-2.
Extracellular ATP (adenosin-5'-triphosphate) markedly increased the intracellular calcium concentration ([Ca^<2+>]_i) the suprachoroid. The first rapid transient increase in [Ca^<2+>]_i was less sensitive to extracellular calcium concentration than the second persistent increase. It was concluded that the first increase in [Ca^<2+>]_i might be mediated by intracellular signla transduction system which liberated Ca^<2+> from intracellular calcium store site and the second persistent phase might be Ca^<2+>-influx from extracellular fluid.
On the other hand, perfusion of a potassium free Krebs solution on the preparation elicited triphasic changes in [Ca^<2+>]_i ; first, calcium ion flowed in from extracellular fluid, second, the increased intracellular calcium was taken up into intracellular organella and third, calcium ion was released from intracellular store sites. The second recovery phase was prolonged by short application of ATP or UTP, prior to the potassium free solution.
These results indicate that the suprachoroid of eye possesses physiological function and plays some role for eye. For further investigation of function of suprachoroid, following pilot studies were performed ; scleral cartilage cells were cultured, interactions with suprachoroid was tested by co-culture and intracellular calcium concentration was measured.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 平井恵二: "外来性ATP,UTP,のIP3産生を介した脈絡上板細胞内Ca^<2+>濃度上昇作用." 眼科紀要. 45(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 申 英蘭: "細胞外アデノシン三燐酸により惹起される細胞内情報伝達系を介したウサギ脈絡上板の細胞内カルシウム濃度上昇." 日本眼科学会雑誌. 98. 69-74 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Midori Funata: "An ultrastructural study of the suprachoroidea." Proc.Current Asp.Ophthalmol.700-703 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 廣瀬 晶: "脈絡上板組織のカルシウムイオン取り込みに対するATPの作用." 眼科紀要. 42. 1385-1392 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiji Hirai: "ATP-activated Ca^<2+>-uptake of the suprachoroid in rabbit eyes." Jpn.J.Physiol.41. S42 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 所 敬: "実験近視研究の現況." 眼科紀要. 42. 1489-1497 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiji Hirai: "Extracellular ATP and UTP increase intracellular calcium concentration via IP3-production in the suprachoroid." Folia Ophthalmol. Jpn.45(in printing). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ying-Lan Shen: "Extracellular adenosine-5'-triphosphate increases intracellular calcium concentration of the rabbit eye suprachoroid via activation of the intracellular signal transduction." J.Jpn. Ophthalmol Soc.98. 69-74 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiji Hirai: "Extracellular ATP increased intracellular calcium concentration in the rabbit eye suprachoroid via a transmenbrane signal transduction system." Jpn. J.Physiol.43. S49 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Midori Funata.: "An ultrastructural study of the suprachoroidea." Proc. Current Asp. Ophthalmol.700-703 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Hirose: "Effect of ATP on Ca^<2+>-uptake in the suprachoroid of the rabbit eye." Folia Ophthalmol. Jpn.42. 1385-1392 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiji Hirai: "ATP-activated Ca^<2+>-uptake of the suprachoroid in rabbit eyes." Jpn. J.Physiol.41. S42 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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