1992 Fiscal Year Final Research Report Summary
Mechanisms involved in bio-mineralization:analyses with chondrocyte cultures.
| Project/Area Number |
03454428
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KATO Yukio Hiroshima Univ. School of Dentistry Professor, 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
ASADA Akira Osaka Univ. School of Dentistry Assistant Professor, 歯学部, 講師 (50028734)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Mineralization / Chondrocytes / Cartilage / Collagen / Proteoglycan |
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| Research Abstract |
In this study, we investigated the mechanisms involved in bio-mineralization using rabbit growth plate chondrocytes in culture. We found that receptors for FGF, 1,25(OH)_2vitamin D_3 decrease and increase, respectively, during the stage of mineralization. Furthermore, the inhibition of type II collagen resulted in suppression of mineralization. However, the inhibition of proteoglycan synthesis or type X collagen synthesis had little effect on the mineralization. The monoclonal antibody 7L62 stained 300k protein which was exclusively located in the matrix of calcifying cartilage. No staining was observed with resting or articular cartilage. We also determined the aminoacid composition of rabbit type X collagen and its amino-terminal amino acid sequence. Various hormones and growth factors suppressed or stimulated this synthesis of type X.
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