1992 Fiscal Year Final Research Report Summary
Role of gap-junctional intercellular communication in salivary secretion and its biochemical mechanism
| Project/Area Number |
03454430
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KANNO Yoshinobu HIROSHIMA UNIVERSITY School of Dentistry, Department of Physiology, Professor, 歯学部, 教授 (00034158)
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| Co-Investigator(Kenkyū-buntansha) |
HIRONO Chikara School of Dentistry, Department of Physiology, Research Associate, 歯学部, 助手 (10199135)
SUGITA Makoto School of Dentistry, Department of Physiology, Research Associate, 歯学部, 助手 (50235884)
SHIBA Yoshiki School of Dentistry, Department of Physiology, Associate Professor, 歯学部, 助教授 (90110452)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Cell communication / Salivary gland / Salivary secretion / Gap junctions / Autonomic nerve |
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| Research Abstract |
The gap-junctional intercellular communication and salivary secretion in rat salivary glands were investigated to clarify the role of gap-junctional communication in the control of salivary secretion.
Fluorescent dye was transferred within 1 min from an acinar cell to the neiboring cells in isolated acini. The gap junctions in the acinar cells was consisted of 27 kDa proteins, and partially 21 kDa proteins. Acetylcholine and carbachol inhibited dye-coupling, and adrenaline and noradrenaline also inhibited the dye-coupling. The inhibition of gap-junction by such autonomic agonists was suppressed by c-kinase inhibitor and calmodulin inhibitor. Activator of c-kinase, 12-O-tetradecanolphorbol-13-acetate, suppressed the dye-coupling.
The secretion of calcein from the free acinar cells was obvious than that from aggregates of acinar cells, but the secretion of amylase from the aggregates of acinar cells was obvious than that from isolated acinar cells.
From these results, the control mechanism of gap junctional communication by c-kinase and calmodulin and the role of gap-junctional communication in the salivary secretion were clarified.
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