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1992 Fiscal Year Final Research Report Summary

ROLES OF PROTEOLYTIC ENZYMES AND LYSOSOMAL MEMBRANE PROTEINS IN BONE RESORBING PROCESS

Research Project

Project/Area Number 03454433
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

YAMAMOTO Kenji  KYUSHU UNIVERSITY DENTAL PHARMACOLOGY PROFESSOR, 歯学部, 教授 (40091326)

Co-Investigator(Kenkyū-buntansha) EZAKI Mitsue  KYUSHU UNIVERSITY DENTAL PHARMACOLOGY, INSTRUCTOR, 歯学部, 助手 (30223647)
KAMATA Osamu  KYUSHU UNIVERSITY DENTAL PHARMACOLOGY, ASSOCIATE PROFESSOR, 歯学部, 助教授 (80037580)
Project Period (FY) 1991 – 1992
KeywordsOSTEOCLAST / BONE RESORPTION / LYSOSOMAL MEMBRANE PROTEIN / CATHEPSIN B / CATHEPSIN L / CATHEPSIN D
Research Abstract

Osteoclastic bone resorption is associated with both the removal of bone mineral and the degradation of bone organic matrix through hydrolytic enzymes secreted by the cells. The present study was designed to investigate the role of lysosomal membrane proteins in the formation of acidic conditions of the bone resorption lacuna and the functional importance of lysosomal proteinases in the bone resorbing process. For this purpose, the levels and localizations of a major 107 kDa lysosomal membrane glycoprotein (designated as LGP107) and lysosomal proteinases (cathepsins B,L and D) in rat osteoclasts were determined by immunocytochemical techniques using antibodies specific for each enzyme. LGP107 was exclusively confined to the apical plasma membrane at the ruffled border of active osteoclasts, where the cells were contact with the bone surface. The ruffled border membrane detached from the bone surface showed a marked decrease in the extent of the immunolabeling. Since LGP107 is highly sialylated and has the very low pI, the protein is likely to contribute to the formation and maintenance of the bone resorption lacuna. Extracellular localization of cathepsins B and L, lysosomal cysteine proteinases, was clearly shown along the bone resorption lacuna and the intracellular immunoreactivity of both cathepsins was weak compared with that of cathepsin D,a lysosomal aspartic proteinase. Cathepsin D was intensely observed in the granules and vacuoles of the osteoclasts, but its extracellular localization was either negligible or not detected along the bone resorption lacuna. These results suggest that these proteinases have their shares of the degradation of the bone matrix.

  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Tetusuya Goto: "Immunocytochemical localization of cathepsin D in the osteoclast." Histochemistry. 97. 13-18 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideak Sakai: "Synthesis and intracellular distribution of cathepsins E and D in differentiating murine Friend erythroleukemia cells." Arch.Biochem.Biophys.294. 412-417 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hudeaki Sakai: "Distribution of two distinct intracellular aspartic proteinases,cathepsins D and E, in rat oral oral tissues as demonstrated by discriminative antibodies." Jpn.J.Oral Biol.34. 317-324 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazushi Kunimatsu: "Possible functions of human neutrophil serine proteinases,medullasin and cathepsin G, in periodontal tissue breakbown." J.Periodont.Res.28. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuya Goto: "Immunohistochemical localization of cathepsisn B,D and L in the rat osteoclast." Histochemistry. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Kiyoshima: "Immunohistocemical localization of cathepsins B and D in the synovial lining cells of the normal rat temporomandibular joint." Arch.Oral Biol.38. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Tsukuba: "Isolation and characterization of recombinant human cathepsin E expressed in Chinese hamster overy cells." J.Biol.Chem.268. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsue Takeda-Ezaki: "Isolation and biochemical characterization of procathepsin E from human erythrocyte membranes." Arch.Biochem.Biophys.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Hara: "Purification of aspartic proteinases from rat epidermis." J.Invest.Dermatol.100. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Nakanishi: "Transient forebrain ischemia induces increased expression and specific localization of cathepsins E and D in rat hippocampus and neostriatum." Exp.Neurol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenji Yamamoto: "Biological Functions of Proteases and Inhibiors" Japan Scientific Societies Press,Tokyo, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 健二: "蛋白分解酵素" 学会出版センター, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 健二: "カテプシンEの生合成と活性制御(ミニレビュー)" 日本生化学会, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Saku, H. Sakai, Y. Shibata, Y. Kato, and K. Yamamoto: "An immunocytochemical study on distinct intracellular localization of cathepsin E and cathepsin D in human gastric cells and various rat cells." J. Biochem. 110, No.6. 956-964 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nakanishi, O.Kamata, K.Ukai, and K.Yamamoto: "Inhibitory effects of NMDA receptor antagonists on hypoxia-induced seizures in dietary Mg^<2+> -deficient mice." Eur. J. Biochem. 204,No.1. 29-34 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Nakanishi, and K. Yamamoto: "NMDA receptor mediated neurotransmission in the basal ganglia and limbic system of the rat. NMDA Receptor Related Agents" Biochemistry, Pharmacology and Behavior (T.Kameyama, T.Nabeshima, and E.F. Domino, eds.) NPP Books, Ann Arbor. pp.141-151 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Sakai, T. Saku, Y. Kato, and K. Yamamoto: "Immunohistochemical and immuno-cytochemical localization of cathepsin E compared with cathepsin D." Structure and Function of the Aspartic Proteinases (B.M. Dunn,ed).) Plenum Pless, New York. pp361-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yamamotol, H. Sakai, E. Ueno, and Y. Kato: "Biological significance and activity control of cathepsin E compared with cathepsin D." Structure and Function of the Aspartic Proteinases (B.M Dunn,ed.) Plenum Press, New York. pp.297-306 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Shindo, S. Koizumi, Y. Kataoka, S. Watanabe, K. Yamamoto, and H. Nakanishi: "Inhibitory effect of NMDA on dopaminergic transmission in a slice preparation of rat globus pallidus." Jpn. J. Pharmacol. 58,No.1. 79-82 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nakanishi, K. Yamamoto, and H. Kita: "Lateral hypothalamus and local stimulation induced postsynaptic responses in zona incerta neurons in an in vitro slice preparation of the rat." Brain Res. 583. 287-291 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Goto, T. Tsukuba, N. Ayasaka, K. Yamamoto, and T. Tanaka: "Immunocytochemical localization of cathepsin D in the rat osteoclast." Histochemistry. 97,No.1. 13-18 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Sakai, Y. Kato, and K. Yamamoto: "Synthesis and intracellular distribution of cathepsins E and D in differentiating murine Friend erythroleukemia cells." Arch. Biochem. Biophys. 294,No.2. 412-417 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Sakai, T. Saku, Y. Kato, and K. Yamamoto: "Distribution of two distinct intracellular sapartic proteinases, cathepsins D and E, in rat oral tissues as demonstrated by discriminative antibodies." Jpn. J. Oral Biol.34,No.3. 317-324 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Tsukuba,H. Hori, T. Azuma, T. Takahashi, R.T. Taggart, A. Akamine, M. Ezaki, H. Nakanishi, H. Sakai, and Yamamoto: "Isolation and characterization of recombinant human cathepsin E expressed in Chinese hamster ovary cells." J. Biol. Chem.268,No.9. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kiyoshima, T. Tsukuba, M.A. Kido, H. Tashiro, K. Yamamoto, and T. Tanaka: "Immunohistochemical localization of cathepsins B and D in the synovial cells of the normal rat temporomandibular joint." Arch. Oral Biol. 38. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Goto, T. Tsukuba, T. Kiyoshima, Y. Nishimura, K. Kato, K. Yamamoto, and T. Tanaka: "Immunohistochemical localization of cathepsins B, D and L in the rat osteoclast." Histochemistry. 99. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takeda-Ezaki, and K. Yamamoto: "Isolation and biochemical characterization of pro-cathepsin E from human erythrocyte membranes." Arch. Biochem. Biophys.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Hara, K. Fukuyama, H. Sakai, K. Yamamoto, and W.L. Epstein: "Purification of aspartic proteinases from rat epidermis." J. Invest. Dermatol.100. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Nakanishi, T. Tsukuba, t. Kondo, T. Tanaka, and K. Yamamoto: "Transient forebrain ischemia induces increased expression and specific localizatoin of cathepsins E and D in rat hippocampus and neostriatum." Exp. Neurol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Kunimatsu, N. Mine, I. Kato, T. Hase, Y. Aoki, and K. Yamamoto: "Possible functions of human neutrophil serine proteinses, medullasin and cathepsin G, in periodontal tissue breakdown." J. Periodont. Res.28. (1933)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Yamamoto, H. Sakai, M. Ezaki, and Y. Kato: "Biosynthesis, processing and localization of cathepsin E." Biological Functions of Proteases and Inhibitors (N.Katunuma, K.Suzuki, J.Travis, and H.Fritz, eds.) Japan Scientific Societies Press, Tokyo. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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