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1993 Fiscal Year Final Research Report Summary

Molecular Basis of Leukocyte Adhesion Molecules in Early-onset Periodontitis Patients.

Research Project

Project/Area Number 03454441
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOkayama University Dental School

Principal Investigator

MURAYAMA Yoji  Okayama Univ. Dental Sch., Professor, 歯学部, 教授 (50029972)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Hideki  Okayama Univ. Dental Sch., Assistant Professor, 歯学部附属病院, 講師 (70170983)
TAKASHIBA Shogo  Okayama Univ. Dental Sch., Assistant, 歯学部附属病院, 助手 (50226768)
TAKAHASHI Keiso  Okayama Univ. Dental Sch., Assistant, 歯学部附属病院, 助手 (70243475)
ISOSHIMA Osamu  Okayama Univ. Dental Sch., Assistant Professor, 歯学部附属病院, 講師 (90176256)
KURIHARA Hidemi  Okayama Univ. Dental Sch., Associate Professor, 歯学部, 助教授 (40161765)
Project Period (FY) 1991 – 1993
Keywordsearly-onset periodontitis / CD11 / CD18 / aggregation / mRNA
Research Abstract

We analyzed the cell-cell adherence related to CD11/CD18 and CD18 mRNA of the individuals with decreased CD11/CD18 expression on the surface of their neutrophils. Epstein Barr virus-transformed B cell lines were developed from one localized juvenile periodontitis (LIP) patient characterized by decreased CD11/CD18 on the neutrophils in peripheral blood and without any remarkable systemic diseases, two siblings with generalized prepubertal periodontitis (GPP) caused from leukocyte adhesion deficiency (LAD), another LIP patient, one localized prepubertal periodontitis (LPP) patient, and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the 32p-labeled CD18 cDNA.The coding region of the mRNA was analyzed by the reverse transcription-polymerase chain reaction method. Base-mismatches between CD18 mRNA and the 32p-labeled RNA probe synthesized from Cd18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LIP patients with decreased CD11/CD18 formed more clusters of smaller size and with fewer cells than those of did the patients and healthy subjects, while the cells from both GPP patients were found not to aggregate and not to form clusters either in the absence or presence of PMA.There were no differences in the length and the amount of mRNA between the LIP patient and the other patients and healthy subjects, while GPP patients expressed a small amount of long mRNA.The whole coding region (2,313 base pairs) was amplified from all of subjects except the GPP Patients, while the 5'-region (1,119 base pairs) was amplified from all subjects. Base-mismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPPpatients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LIP patient with an anom

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 村山洋二: "歯周病の最前線-歯周病の遺伝子診断" 日本歯科医師会雑誌. 46. 555-565 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Katsuragi,S.Takashiba,H.Kurihara,Y.Murayama: "Molecular basis of leukocyte adhesion molecnles in early-onset periodontitis patients" J.Periodontol.(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Takahashi et al.: "Role of cytokine in the induction of adhesion molecules on cultured human gingival fibroblasts" J.Periodontol.65(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Takahashi et al.: "The inhibition of interferon-γ-induced upregulation of HLA-DR antigen expression on cultured human gingival fibroblast by IL-1β or TNFα" J.Periodontol.65(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Takashiba et al.: "Unique intronic variations of HLA-DQβ gene in early-onset periodontitis" J.Periodontol.65(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Murayama,K.Katsuragi,S.Takashiba,H.Kurihara: "Molecular Pathegenesis of Periodontal Disease" R.J.Genco(ed) ASM, (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoji MURAYAMA: "The front in periodontal disease--Genetic diagnosis" J.Japan Dent.Ass.46. 555-565 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyoko KATSURAGI, Shogo TAKASHIBA, Hidemi KURIHARA, Yoji MURAYAMA: "Molecular basis of leukocyte adhesion molecules in early-onset periodontitis patients" J.Periodontol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiso TAKAHASHI et al.: "Role of cytokine in the induction of adhesion molecules on cultured human gingival fibroblasts" J.Periodontol.65 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiso TAKAHASHI et al.: "The inhibition of interferon-gamma-induced upregulation of HLA-DR antigen expression on cultured human gingival fibroblast by IL-1beta or TNFalpha" J.Periodontol.65 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shogo TAKASHIBA et al.: "Unique intronic variations of HLA-DQbeta gene in early-onset periodontitis" J.Periodontol.65 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoji MURAYAMA, Kyoko KATSURAGI, Shogo TAKASHIBA, Hidemi KURIHARA: "Molecular Pathogenesis of Periodontal Disease" R.J.Genco(ed) ASM. 18 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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