1993 Fiscal Year Final Research Report Summary
The Study of Mechamism of Calcification and Differentiation of an Osteoblastic Clonal Cell Line from Human Mandibular Osteosarcoma
| Project/Area Number |
03454470
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
YOSHIKAWA Hiromasa KYUSHU UNIVERSITY Faculty of Dentistry, Associate Researcher, 歯学部, 助手 (00220608)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Hideo KYUSHU UNIVERSITY Faculty of Dentistry, Former Professor, 歯学部, 元教授 (20037500)
OHISHI Masamich KYUSHU UNIVERSITY Faculty of Dentistry, Professor, 歯学部, 教授 (70037505)
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| Project Period (FY) |
1991 – 1993
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| Keywords | human mandibular osteosarcoma cell line / osteoblastic cell / ALP activity |
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| Research Abstract |
A human mandibular osteosarcoma cell line with osteoblastic phenotypes and tumorgenicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture. Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end of logarithmic cell growth than at the resting stage. 1, 25 (OH) 2D3 also stimulated the ALP activity, but its effect was low and was not differentin any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumors to the original one, with abundant osteoid were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the this cell line expresses an immature preosteoblastic phenotype. Because of these characteristics, this cell line should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and etiology of human osteosarcoma of the jaw.
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