1993 Fiscal Year Final Research Report Summary
HSP90, a stress protein which regulates functional proteins
| Project/Area Number |
03454541
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
YAHARA Ichiro Tokyo Met. Inst. Med. Sci., Vice-Dir., 副所長 (60109957)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Yoshihiko TMIMS.Dept. Cell. Biol., Res. Sci., 細胞生物学研究部門, 研究員 (70209914)
IIDA Kazuko TMIMS.Dept. Cell. Biol., Res. Sci., 細胞生物学研究部門, 研究員 (40151229)
MATSUMOTO Seiji TMIMS.Dept. Cell. Biol., Res. Sci., 細胞生物学研究部門, 研究員 (40190532)
MINAMI Yasufumi TMIMS.Dept. Cell. Biol., Res. Sci., 細胞生物学研究部門, 研究員 (40181953)
MIYATA Yoshihiko TMIMS.Dept. Cell. Biol., Res. Sci. (70209914)
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| Project Period (FY) |
1991 – 1993
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| Keywords | stress proteins / HSP90 / calmodulin-binding domain / dimerization domain / molecular chaperone / casein kinase II / budding yeast / temperature-sensitive mutants |
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| Research Abstract |
1) Domain structure of HSP90 : The calmodulin-binding domain of HSP90 was identified by cross-linking of HSP90 and Ca-calmodulin with a chemical cross-linker, EDC, followed by CNBr treatment. The domain consists of 21 amino acid residues which fold into an amphiphilic alpha-helix, This identification was confirmed by chemically syntheizing the paptide and examining its binding ability to calmodulin. Next, proteolytic cleavage of C-terminus of HSP90 was expressed in reticulocyte lysate, it formed dimer. When a mutated HSP90 lacking of its C-terminal 49 amino acids was expressed, no dimer was formed. These results suggest that the calboxy-terminal region is necessary for its dimerization.
2) We found that HSP90 protects casein kinase II from self-aggregation and inactivation.
3) We isolated five HSP90 ts-mutants of the budding yeast, Saccharomyces cerevisiae. Phenotypes expressed at the non-permissive temperature were extensively analyzed.
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Research Products
(12 results)
