1992 Fiscal Year Final Research Report Summary

In Vitro Motility Assays of Myosin Blocked with Modifiers

Research Project

Project/Area Number	03454555
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	分子遺伝学・分子生理学
Research Institution	Asahikawa Medical College
Principal Investigator	HIRATSUKA Toshiaki Asahikawa Medical College Chemistry Associate Professor, 医学部, 助教授 (30041825)
Project Period (FY)	1991 – 1992
Keywords	Myosin / Muscle contraction / Motility assays
Research Abstract	In Vitro Motility Assays of Myosin Blocked with Modifiers The effects of the chemical modifications of myosin's reactive cysteines on actomyosin adenosine triphosphatase(ATPase) activities and sliding patterns in the in vitro motility assays were examined. The two types of modifications studied were 5-(iodoacetamido)fluorescein labeling of SH1(Cys-707) and 2-(4'- maleimidylanilino)naphthalene-6-sulfonic acid labeling of SH2(Cys-697). Each type of modified myosin inhibited the sliding of actin in motility assays. However, the possibility that the observed inhibition of the sliding of actin induced by modified myosins may result from structural perturbation caused by bulky modifiers cannot be ruled out by the present work.