1992 Fiscal Year Final Research Report Summary
Construction and expression of gene families
| Project/Area Number |
03454561
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Fujita Health University |
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Principal Investigator |
KUROSAWA Yoshikazu Fujita Health Univ., Comprehensive Med. Sci., Professor, 総合医科学研究所, 教授 (10109259)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Kazuhiro Fujita Health Univ. College, , Assistant, 助手 (80132257)
OKAMURA Kazuhiko Fujita Health Univ. College, , Assistant, 助手 (60132255)
MATSUI Toshikazu Fujita Health Univ., Comprehensive Med. Sci., Assistant Professor, 医学部, 講師 (50181681)
HASHIMOTO Keiichiro Fujita Health Univ., Comprehensive Med. Sci., Assistant Professor, 総合医科学研究所, 講師 (70192268)
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| Project Period (FY) |
1991 – 1992
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| Keywords | MHC / Complement / Immune system / Homeobox / Sponge |
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| Research Abstract |
It has been believed that the most primitive animal that produces antibodies is cyclostome. We isolated the gene which encodes hagfish "antibodies" and determined the nucleotide sequence. Surprisingly, it turned out to be a gene encoding not immunoglobulin but complement C3. We developed a new method using PCR for isolation of MHC genes from primitive vertebrates. By this method, we have succeeded to isolate genes encoding MHC classI and classIIbeta from carp and also a gene encoding MHC classI from shark. We also showed that at the level of bony fish MHC classI genes have been expanded to at least several tens and their sequences are quite heterogeneous.
We examined homeobox-containing genes from various phyla of animals. We isolated two homeobox genes, prox1 and prox2 from sponge. The structure of prox1 and prox2 indicated that they have gained the properties characteristics for each class of homeobox genes isolated from higher animals. We isolated four homeobox genes from hydra. A new kind of homeobox gene was identified from C. elegans. Two kinds of new homeobox genes, GBX1 and GBX2, were isolated from human. We mapped GBX1 to chromosome 7q36.1.
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