1992 Fiscal Year Final Research Report Summary
Development of a bead-ELISA kit for rapid identification of enteric bacteria
| Project/Area Number |
03557023
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKEDA Yoshifumi Faculty of Medicine, Kyoto University, Proffessor, 医学部, 教授 (30029772)
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| Co-Investigator(Kenkyū-buntansha) |
OKU Yuichi Nissui Pharmaceut.Co., Director, 開発研究部, 研究員
UESAKA Yoshihiko nissui Pharmaceut.Co., Director, 開発研究部, 副主任研究員
HONDA Tetsuro Nissui Pharmaceut.Co., Director, 中央研究所, 所長
KURAZONO Hisao Faculty of Medicine, Kyoto University, Assistant, 医学部, 助手 (90186487)
NISHIBUCHI Mitsuaki Faculty of Medicine, Kyoto University, Associate Professor, 医学部, 助教授 (50189304)
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| Project Period (FY) |
1991 – 1992
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| Keywords | bead-ELISA / cholera toxin / Campylobacter jejuni |
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| Research Abstract |
To diagnose bacterial infection, it is necessary to identify the causative bacteria as early as possible. However, conventional methods to identify bacteria by using culture methods takes at shortest 20-30 hours after obtaining clinical materials.
To evaluate a recently developed highly sensitive bead-ELISA to be used for a tool for the rapid identification of the causative bacteria of enteric infection, we cooperate with Institute of Cholera and Enteric Diseases of India and applied the bead-ELISA for stool specimens of cholera patients. The results showed that the bead-ELISA for cholera toxin is a rapid and sensitive method to detect cholera toxin, thus to diagnose cholera patient.
Bead-ELISA system was also developed by using a specific antigen and whole cell antigen to compylobacter jejuni. It was demonstrated that the a developed bead-ELISA is useful to identify Campylobacter jejuni. Thus an attempt will be made to develop a kit for identification of Campylobacter jejuni.
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[Publications] T.Ramamurthy, Amit Pal, M.K. Bhattacharya, S.K.Bhattacharya, A.Saha Chowdhury, Yoshifumi Takeda, Tae Takeda, S.C.Pal and G.Balakrish Nair: "Serovar, biotype, phagetype, toxigenicity and antibiotic susceptibility patterns of Vibrio cholerae isolated during two consecutive cholera seasons (1989-90) in Calcutta." Indian J.Med. Res. A 95. 125-129 (1992)
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