1993 Fiscal Year Final Research Report Summary
Gross culture system for cyanobacteria & its application for medication
| Project/Area Number |
03557024
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Jichi Medical School |
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Principal Investigator |
NAKANO Masayasu Jichi Medical School, Dept.Microbiol., Professor, 医学部, 教授 (70048958)
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| Co-Investigator(Kenkyū-buntansha) |
KIRIKAE Teruo Jichi Med.Sch., Dept.Microbiol., Instructor, 医学部, 助手 (50192563)
SHIRAI Makoto Ibaraki Univ., School of Agriculture, Associ.Prof., 農学部, 助教授 (10007792)
TAKI Tatuo Kitasato University, School of Hygiene, Associ.Prof., 衛生学部, 助教授 (70049097)
SAITO Shinji Jichi Med.Sch., Dept.Microbiol., Instructor, 医学部, 助手 (50195989)
MATUURA Motohiro Jichi Med.Sch., Dept.Microbiol., Associate Prof., 医学部, 助教授 (20150089)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Cyanobacteria / Microcystin / Microcystis aeruginosa / antibacterial substance / culture method / Interleukin-6 / monoclonal antibody / gamma-linolenic acid |
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| Research Abstract |
A gross culture system for microcystis (cyanobactera) has been developed. When the cells of Microcystis aeruginosa K-139 strain were inoculated (10% v/v) into the system with 10 litters of CB medium (pH 10.0) and were cultured at 28C。 under the conditions of airing (filter-passed air, 9 liters/min) and lighting (4 directions, cool white light, 10, 000 lux). The pH of medium was maintained between 9 and 10, by being adjusted with 1 N HCl solution during the culture period. The cell-growth reached the maximum at day 7, and the yield of cells was 4.56 g. It was 2.5 times greater than that obtained from a stationary culture. The yield of microcystin extracted from the cell-mass was 27.493 mg, and it was 3 times more than that of the statinary culture.
Monoclonal antibodies against microcystin-LR were prepared from hybridoma cell lines. All the antibodies were reacted with not only microcystin-LR but also other microcystins tested. Microcystin-shock in mice could hardly be protected by a previous administration of these antibodies. The microcystin-shock was relieved to some extent when interleukin (IL)-10 was injected 30 min in advance. The serum-level of IL-6 induced by microcystin was lowered by a previous injection of IL-10.
The cell-extract of Microcystis aeruginosa K-18 strain contained anti-bacterial activity against various kinds of gram-positive and negative bacteria. The active substance was purified. However, it was found to be gamma-linolenic acid.
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