• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

Creation of human parvovirus B19 antigen for diagnostic use.

Research Project

Project/Area Number 03557025
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

NUNOUE Tadasu  Kyushu Univ.Sch of Health Sciences Prof, 医療技術短期大学部, 教授 (20038649)

Co-Investigator(Kenkyū-buntansha) UMEMURA Tsukuru  Kyushu Univ.Sch of Health Sciences Prof, 医療技術短期大学部, 教授 (90136432)
UMENE Kenichi  Kyushu Univ.Fac of Medicine Assoc Prof, 医学部, 助教授 (70127984)
Project Period (FY) 1991 – 1993
KeywordsParvovirus B19 / Antigenic Epitope / Synthetic Peptides / Cell Culture
Research Abstract

Aims ; To create human parvovirus B19 antigens.
Methods ; Productions of virus particles in cell cultures, of antigenic protein by gene engineering, and of antigenic peptides by synthesis.
Results ; Among the hematopoietic cell lines ; K562, KU-812-F, RO 10 and JK-1, and mononuclear cells in umbilical cord blood and in venous blood (rest of the component blood transfusion), virus grew better in mononuclear cells from umbilical cord blood, though less than in those from bone marrow cells. Virus protein in e.coli was hardly produced by almost full length of the genome DNA cut bilateral ends with a restriction enzyme EcoR 1. Novel five synthetic peptides were showed clear and specific antigenicity by indirect ELISA test, better than two, which were 3293 - 3364 (reported by Fridell), 4106- 4156 (reported by Sato). Gene positions of those were 2912 - 2956, 2892 - 2971 in the region of VP1, 4016 - 4081, 4142 - 4189, and 4127 - 4201 in VP2. The full paper is in preparation.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 布上 董: "ヒトパルボウイルスB19感染症の臨床像と診断法の問題" 臨床とウイルス. 22(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umene K,Nunoue T: "Partial nucleotide sequencing and charecterization of human parvovirus B19 genome DNAs from damaged human fetuses and from patients with leukemia." J Med Virol. 39. 333-339 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 布上 董: "パルボウイルスB19抗体測定用ELISA(IBL製)の検討" 臨床とウイルス. 20. 424-428 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 布上 董: "ヒトパルボウイルスB19関連疾患の遺伝子診断" 日本臨床. (642). 222-226 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nunoue T,Umene K: "Study of diagnosis for fetal infection with human parvovirus B19" Proceedings The second Fukuoka international symposium on perinatal medicine. (2). 87-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 布上 董(斎藤 厚・島田 馨編): "最新内科学大系26(ウイルス感染症・パルボウイルスB19)" 中山書店, 11/318 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nunoue T: "Clinical manifestations and diagnostic problems of parvovirus B19 infections. (in Japanese)" Clinical Virology. 22(1) ; (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umene K, Nunoue T: "Partial nucleotide sequencing and characterization of huma parvovirus B19 genome DNAs from damaged human fetuses and from patients with leukemia." J Med Virol. 39. 333-339 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nunoue T: "Test of newly developed ELISA (IBL) for anti-parvovirus B19 IgG and IgM detection. (in Japanese)" Clinical Virology. 20(5). 424-428 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nunoue T, Umene K: "Study of diagnosis for fetal infection with human parvovirus B19." Proceedings ; The second Fukuoka International Symposium on Perinatal Medicine. (2). 87-91 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nunoue T: "Molecular diagnosis of Human parvovirus B19 infections. (in Japanese)" Nippon rinsho. (642). 222-226 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi