1993 Fiscal Year Final Research Report Summary
Creation of human parvovirus B19 antigen for diagnostic use.
Project/Area Number |
03557025
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Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | KYUSHU UNIVERSITY |
Principal Investigator |
NUNOUE Tadasu Kyushu Univ.Sch of Health Sciences Prof, 医療技術短期大学部, 教授 (20038649)
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Co-Investigator(Kenkyū-buntansha) |
UMEMURA Tsukuru Kyushu Univ.Sch of Health Sciences Prof, 医療技術短期大学部, 教授 (90136432)
UMENE Kenichi Kyushu Univ.Fac of Medicine Assoc Prof, 医学部, 助教授 (70127984)
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Project Period (FY) |
1991 – 1993
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Keywords | Parvovirus B19 / Antigenic Epitope / Synthetic Peptides / Cell Culture |
Research Abstract |
Aims ; To create human parvovirus B19 antigens. Methods ; Productions of virus particles in cell cultures, of antigenic protein by gene engineering, and of antigenic peptides by synthesis. Results ; Among the hematopoietic cell lines ; K562, KU-812-F, RO 10 and JK-1, and mononuclear cells in umbilical cord blood and in venous blood (rest of the component blood transfusion), virus grew better in mononuclear cells from umbilical cord blood, though less than in those from bone marrow cells. Virus protein in e.coli was hardly produced by almost full length of the genome DNA cut bilateral ends with a restriction enzyme EcoR 1. Novel five synthetic peptides were showed clear and specific antigenicity by indirect ELISA test, better than two, which were 3293 - 3364 (reported by Fridell), 4106- 4156 (reported by Sato). Gene positions of those were 2912 - 2956, 2892 - 2971 in the region of VP1, 4016 - 4081, 4142 - 4189, and 4127 - 4201 in VP2. The full paper is in preparation.
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