1993 Fiscal Year Final Research Report Summary
Developmental studies on practical use of ABO blood typing using immunoelectrodes
| Project/Area Number |
03557030
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
FURUKAWA Ken Gunma Univ.Sch.Med.Dept.of Legal Med.Prof., 医学部, 教授 (90008227)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Tamiko Gunma Univ.Sch.Med.Dept.of Legal Med.Assistant, 医学部, 助手 (40008561)
MATSUKI Takasume Gunma Univ.Sch.Med.Dept.of Legal Med.Assistant Prof., 医学部, 助教授 (10126617)
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| Project Period (FY) |
1991 – 1993
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| Keywords | ABO blood groups / Blood group determination / Electrode / Membrane potential / Potential |
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| Research Abstract |
1) A titanium wire 1 mm in diameter was heated at ca.1000。C to make a thin film of oxide on the surface. The tip of the wire was immersed in a 2 mg/ml solution of cyanogen bromide pH11 for 20 min, washed and combined with anti-A, anti-B and eel anti-H antibody at pH 8.5 for 20 min. The electrode was sealed with Teflon. The reference electrode was prepared in the same manner except treatment with antibody. After the electrodes were set in the PBS with stirring, the corresponding human red cells or blood group substance was added to the PBS.A change in potential towards positive was observed after the addition of antigens. Addition of non-corresponding red cells or blood group substances did not change the potential. The result indicated that the ABO blood group determinations of red cells or blood group substances were possible using antibody fixed electrodes.
2) Human blood group stroma from blood group O, A and B red cell was fixed in a triacetyl-cellulose membrane. Electrochemical blood typing of membrane stroma was performed with antisera, using a pair of membranes with immobilized H, A or B stroma and stroma unfixed control membrane. The membrane-bound blood group substance retained its binding ability to the corresponding antibody in antiserum. The transmembrane potential changed after the antibody binding reactions with the membrane-fixed blood group substance. The blood group was determined measuring the membrane potential across these membranes before and after the antigen-antibody reaction.
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