1992 Fiscal Year Annual Research Report

遺伝子増幅法によるエンテロウイルス診断法の開発

Research Project

Project/Area Number	03557042
Research Institution	KYOTO UNIVERSITY
Principal Investigator	松森昭京都大学, 医学部, 講師 (70135573)
Keywords	遺伝子増幅法 / ポリメラーゼ連鎖反応 / エンテロウイルス / コクサッキーウイルス / 心筋炎 / 心筋症 / カルディオウイルス / ピコルナウイルス
Research Abstract	平成3年度の研究成果をもとに本年度はヒト心筋症、心筋炎症例の心筋生検、剖検標本を用いてエンテロウイルスPCRを行った。心筋炎16例、拡張型心筋症9例、他の心疾患2例を対象として心筋生検及び剖検標本よりRNAを抽出後、ランダムプライマーでcDNAを作製し、多くのエンテロウイルス遺伝子の5'末端領域を増幅するプライマー及びウイルスカプシド領域の中でコクサッキーウイルスB3特異的領域を増幅することが可能な2種類のプライマーを用いてPCRを行った。27例の心筋標本より10年以上病歴を有する拡張型心筋症の1例から多数エンテロウイルス遺伝子の5'末端に対応する195塩基の領域を増幅することができ、さらに、同症例よりコクサッキーウイルスB3の特異領域も検出できた。また、コクサッキーウイルスと同様に心筋親和性の高いピコルナウイルスであるencephalomyocarditis(EMC)ウイルスについてもPCR法を開発した。プライマーは、5'非翻訳領域を用いた。培養細胞を用いた検討では、EMCウイルス100コピーを検出でき、高感度であることが判明した。DBA/2マウスにおけるEMCウイルス性心筋炎モデルでの検討では、従来の方法によるウイルス分離が陰性となった後もPCR法では3ヶ月後の長期にわたりウイルスRNAが検出できることが明かとなった。本研究により開発されたPCR法によるエンテロウイルスを含むピコルナウイルスRNAの検出法は従来のウイルス検出法に比し、短時間かつ簡便で感度も高く、臨床的に極めて有用であると考える。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Hosono M: "Human/mouse chimeric antibodies show low reactivity with human anti-murine antibodies(HAMA)." Br J Cancer. 65. 197-200 (1992)
[Publications] Matsumori A: "Lessons from animal experiments in myocarditis." Herz. 17. 107-111 (1992)
[Publications] Okada I: "Successive infection of coxsackievirus B3 and encephalomyocarditis virus:An animal model of chronic myocarditis." J Pathol. 167. 341-347 (1992)
[Publications] Sato Y: "Effect of immunostimulant therapy on acute viral myocarditis in an animal model." Am Heart J. 124. 428-434 (1992)
[Publications] Yamada T: "The effect of α_1-blocker,bunazosin on a murine model of congestive heart failure induced by viral myocarditis." Jpn Circ J. 56. 1138-1145 (1992)
[Publications] Okada I: "Combination treatment with ribavirin and interferon for experimental coxsackievirus B3 replication." J Lab Clin Med. 120. 569-573 (1992)
[Publications] Horike K: "Relation between myoglobin and cardiac dysfunction in myocarditis:immunohistochemical study of endomyocardial biopsy specimens." Jpn Circ J. 55. 24-32 (1991)
[Publications] Matsumori A: "Pathogenesis of myocardial injury in myocarditis and cardiomyopathy." Jpn Circ J. 55. 1132-1137 (1991)
[Publications] Matsumori A: "The effect of beta-adrenergic blockade in dilated cardiomyopathy A questionnaire study in Japan." Heart and Vessels. Suppl. 6-10 (1991)
[Publications] Okada I: "Detection of viral RNA in experimental coxsackievirus B3 myocarditis of mice using the polymerase chain reaction." Int J Exp Pathol. 73. 721-731 (1992)
[Publications] Kyu B: "Cardiac persistence of cardioviral RNA detected by polymerase chain reaction in a murine model of dilated cardiomyopathy." Circulation. 86. 522-530 (1992)
[Publications] Sato Y: "Treatment of coxsackievirus B3 myocarditis by immunoactive peptide in an animal model." Clin Immunol Immunopathol. 65. 65-69 (1992)
[Publications] Yamada T: "Time-course of myocardial infarction evaluated by indium-111 antimyosin monoclonal antibody scintigraphy:Clinical implications and prognostic value." J Nucl Med. 33. 1501-1508 (1992)
[Publications] Tominaga M: "Activation of Ca-permeable cation channels by myocarditis-associated antibody in guinea pig ventricular myocytes." J Clin Invest.
[Publications] Suzuki H: "Enhanced expression of superoxide dismutase messenger RNA in viral myocarditis:An SH dependent reduction of its expression and myocardial Injury." J Clin Invest.
[Publications] Matsumori A: "A questionnaire study of β-adrenergic blockade in dilated cardiomyopathy in Japan." Jpn Circ J. 56. 767-775 (1992)
[Publications] Anan R: "Cardiomyopathy update 6.Molecular genetic analyses of familial hypertrophic cardiomyopathy." University of Tokyo Press,
[Publications] Matsumori A: "In Viral infections of the heart.Experimental studies." Edward Arnold,Kent England,
[Publications] Matsumori A: "Mechanism and significance of myocardial uptake of antimyosin antibody in myocarditis and cardiomyopathy:Clinical and experimental studies." Springer-Verlag,
[Publications] Matsumori A: "International Symposium on ldiopathic Dllated Cardiomyopathy.Animal models for therapeutic trials of viral myocarditis." Springer-Verlag,

1992 Fiscal Year Annual Research Report

遺伝子増幅法によるエンテロウイルス診断法の開発

Principal Investigator

松森 昭 京都大学, 医学部, 講師 (70135573)

Research Products

[Publications] Hosono M: "Human/mouse chimeric antibodies show low reactivity with human anti-murine antibodies(HAMA)." Br J Cancer. 65. 197-200 (1992)

[Publications] Matsumori A: "Lessons from animal experiments in myocarditis." Herz. 17. 107-111 (1992)

[Publications] Okada I: "Successive infection of coxsackievirus B3 and encephalomyocarditis virus:An animal model of chronic myocarditis." J Pathol. 167. 341-347 (1992)

[Publications] Sato Y: "Effect of immunostimulant therapy on acute viral myocarditis in an animal model." Am Heart J. 124. 428-434 (1992)

[Publications] Yamada T: "The effect of α_1-blocker,bunazosin on a murine model of congestive heart failure induced by viral myocarditis." Jpn Circ J. 56. 1138-1145 (1992)

[Publications] Okada I: "Combination treatment with ribavirin and interferon for experimental coxsackievirus B3 replication." J Lab Clin Med. 120. 569-573 (1992)

[Publications] Horike K: "Relation between myoglobin and cardiac dysfunction in myocarditis:immunohistochemical study of endomyocardial biopsy specimens." Jpn Circ J. 55. 24-32 (1991)

[Publications] Matsumori A: "Pathogenesis of myocardial injury in myocarditis and cardiomyopathy." Jpn Circ J. 55. 1132-1137 (1991)

[Publications] Matsumori A: "The effect of beta-adrenergic blockade in dilated cardiomyopathy A questionnaire study in Japan." Heart and Vessels. Suppl. 6-10 (1991)

[Publications] Okada I: "Detection of viral RNA in experimental coxsackievirus B3 myocarditis of mice using the polymerase chain reaction." Int J Exp Pathol. 73. 721-731 (1992)

[Publications] Kyu B: "Cardiac persistence of cardioviral RNA detected by polymerase chain reaction in a murine model of dilated cardiomyopathy." Circulation. 86. 522-530 (1992)

[Publications] Sato Y: "Treatment of coxsackievirus B3 myocarditis by immunoactive peptide in an animal model." Clin Immunol Immunopathol. 65. 65-69 (1992)

[Publications] Yamada T: "Time-course of myocardial infarction evaluated by indium-111 antimyosin monoclonal antibody scintigraphy:Clinical implications and prognostic value." J Nucl Med. 33. 1501-1508 (1992)

[Publications] Tominaga M: "Activation of Ca-permeable cation channels by myocarditis-associated antibody in guinea pig ventricular myocytes." J Clin Invest.

[Publications] Suzuki H: "Enhanced expression of superoxide dismutase messenger RNA in viral myocarditis:An SH dependent reduction of its expression and myocardial Injury." J Clin Invest.

[Publications] Matsumori A: "A questionnaire study of β-adrenergic blockade in dilated cardiomyopathy in Japan." Jpn Circ J. 56. 767-775 (1992)

[Publications] Anan R: "Cardiomyopathy update 6.Molecular genetic analyses of familial hypertrophic cardiomyopathy." University of Tokyo Press,

[Publications] Matsumori A: "In Viral infections of the heart.Experimental studies." Edward Arnold,Kent England,

[Publications] Matsumori A: "Mechanism and significance of myocardial uptake of antimyosin antibody in myocarditis and cardiomyopathy:Clinical and experimental studies." Springer-Verlag,

[Publications] Matsumori A: "International Symposium on ldiopathic Dllated Cardiomyopathy.Animal models for therapeutic trials of viral myocarditis." Springer-Verlag,

松森昭京都大学, 医学部, 講師 (70135573)