Mass Screening Procedure for Mucopolysaccharidoses using urine specimens on paper

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 03557044
• Research Category: Grant-in-Aid for Developmental Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Gifu University
• Principal Investigator: ORII Tadao Gifu University. Pediatrics. professor, 医学部, 教授 (20045339)
• Co-Investigator(Kenkyū-buntansha): SUKEGAWA Kazuko Gifu University. Pediatrics. Instructor, 医学部, 助手 (60115409) ; SUZUKI Yasuyuki Gifu University. Pediatrics. Assistant Professor, 医学部・附属病院, 講師 (00163014) ; YAMAGUCHI Seiji Gifu University. Pediatrics. Assistant Professor, 医学部・附属病院, 講師 (60144044)
• Project Period (FY): 1991 – 1993
• Keywords: Inherited Metabolic Disease / Mucopolysaccharidosis / Mass Screening / DMB Method / Urinary Mucopolysaccharide

Research Abstract

We examined the direct quantitative method with 1,9-dimethylmethylene blue (DMB).
1. This method of quantifying excessive excretion of sulfated glycosaminoglycanes was suited to mass screening programs for mucopolysaccharidoses diseases.
2. It required only 12 ul of urine, and many speciments can be assayd in a short time.
3. Test results of the DMB method correlated with those of the CPC/carbazole-borate method.
4. A component in the diaper was highly reactive in the assay.
With this method, we anticipate that all newborns could be tested for MPS disease on a regular basis.