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1993 Fiscal Year Final Research Report Summary

ELECTRICAL AND CHEMICAL STIMULATION OF SPINAL CORD FOR TREATMENT OF URINARY DISTURBANCE

Research Project

Project/Area Number 03557068
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionDEPT.OF UROLOGY, AKITA UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

TSUCHIDA Seigi  Akita Univ., Dept.of Urology, Professor, 医学部, 教授 (00004549)

Co-Investigator(Kenkyū-buntansha) NOTO Hiromitsu  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 助手 (60164711)
NISHIZAWA Osamu  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 講師 (60091815)
HARADA Tadashi  Akita Univ., Dept.of Urology, Assistant Prof., 医学部, 講師 (00108953)
OGAWA Tetsurou  Akita Univ., 1st Dept.of Physiology, Professor, 医学部, 教授 (80004555)
Project Period (FY) 1991 – 1993
Keywordsmicturition / electrical stimulation / chemical stimulation / spinal cord / alpha-adrenergic mechanism / GABArgic mechanism / opiate / nitric oxide
Research Abstract

For the purpose of evaluation of the chemical stimulation of sacral spinal cord to modulate the micturition reflex, intrathecal administration of various drugs was performed using decerebrate dogs. GABA (0.1-0.3 mg), muscimol (0.01-0.2 mg), bacrofen (0.01-0.3 mg) increased bladder capacity 65%, 142%, 95% respectively. Phaclofen did not have a significant effect. Picrotoxin (0.05-0.3 mg) inhibited the effect of muscimol, and picrotoxin alone decreased bladder capacity 28%. These results indicate that there are both GABA-A and -B receptors in the sacral spinal cord, and that intrinsic GABA acts though GABA-A receptor. Intrathecal administration of phenylephrine (0.01-0.43 mg) increased bladder capacity and external urethral sphincter activity. Prazocin (0.01-0.3 mg) did not change bladder activity but decreased external urethral sphincter activity. Clonidine (0.07-0.32 mg) did not change bladder activity but decreased external urethral sphincter activity. Yohimbine (0.07-0.45 mg) decreas … More ed bladder contraction pressure. These results suggest that alpha-adrenergic mechanism in the sacral spinal cord increases bladder capacity and increases the external urethral sphincter activity through alpha-1 receptor, and increases bladder contractility and decreases external urethral sphincter activity through alpha-2 receptor.
Electrical stimulation of the sacral cord as well as sacral roots induced both bladder and urethral sphincter contractions. Bladder contraction was dominant when S-2 was stimulated while external urethral sphincter contraction was dominant when S-1 was stimulated. Potable stimulator (2.5 x 6 x 9 cm, 10-50 Hz, 0.2-1.0 msec duration, 0-50 V) was made for functional electrical stimulation. Electrical stimulation in the pontine micturition center (PMC) induced urethral relaxation as well as bladder contraction. Internal urethral sphincter relaxation was not inhibited by propranolol or atropine, but was abolished by hexamethonium or methylene blue. L-arginine increased bladder capacity and decreased bladder contraction pressure. These responses were partially reversed by methylene blue. Methylene blue enhanced bladder contraction evoked by PMC stimulation and induced spontaneous bladder contraction. These results indicate that internal urethral sphincter relaxations in mediated by non-adrenergic and non-cholinergic mechanism, and that L-arginine - NO pathway has a role in controlling the micturition reflex. Less

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Noto,H.et al.: "Internal urethral sphincter relaxation mediated by a non-adrenergic and non-cholinergic mechanism in decerebrate cats." J.Urol.149. 341A (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoda,N.et al.: "Role of sacral alpha-adrenoceptive mechanisms in micturition reflex in the decerebrate dog." Proc.23rd ICS. 23. 108 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Noto,et al.: "Effect of L-arginine and methylene blue on the micturition reflex in decerebrate dogs." Proc.23rd ICS. 23. 99 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤敬悦: "橋排尿中枢による内尿道括約筋調節機構に関する研究" 日泌尿会誌. 84. 497-506 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋徳男: "除脳イヌの反射性排尿におけるα-アドレナリン作動性機構に関する研究" 日泌尿会誌. 84. 711-719 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西沢理: "脊髄内排尿機構とその障害" 排尿障害プラクティス. 1. 69-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 土田正義: "熊本悦明編,21世紀への泌尿器科学の展望,神経泌尿器科" ライフメディコム, 253(p102-111) (1993)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tsuchida, S.: "Neurourology." Jap.J.Urol.81. 2007-2009 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugaya, K., Nishizawa, O., Noto, H., Tsukada, T., Kohama, T., Shimoda, N.: "Identification of the regions of the pons that repond to baclofen, inaperisone and oxybutynin, which inhibit the micturition reflex." The autonomic nervous system. 28. 513-521 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugaya, K., Nishizawa, O., Noto, H., Tsukada, T., Kohama, T., Shimoda, N., Harada, T.and Tsuchida, S.Morikawa, K.: "Identification of effective region of the pons in response to inaperisone which facilitates urine storage." Acta Urol.Jpn.37. 1639-1644 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizawa, O., Sugaya, K., Shimoda, N., Otomo, R.and Tsuchida, S.: "Role of the nucleus reticularis pontis oralis in the dorsolateral pontine tegmentum on micturition of cat." Neurourol.Urodyn.10. 363-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noto, H., Roppolo, J.R., de Groat, W.C., Nishizawa, O., Sugaya, K.and Tsuchida, S.: "Opiate modulation of the micturition reflex at level of the pontine micturition center." Urol.Int.47(Suppl.1). 19-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsukada, T., Noto, H., Nishizawa, O.and Tsuchida, S.: "Gabanergic modulation of the micturition reflex at the level of the sacral spinal cord in decerebrate dogs." Neurouro.Urodyn.10. 366-367 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noto, H.: "Inhibitory modulation of the micturition." Jap.J.Urol.83. 808-811 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noto, H., Nishizawa, O.and Tsuchida, S.: "Micturition. -functional anatomy and physiology-" Brain Nursing. 8. 865-871 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohama, T.: "Neuroanatomical studies on the urine storage facilitatory areas in the cat brain. Part I.Input teuronal structures to the nuleus locus subcoeruleus and the nucleus reticularis pontis oralis." Jap.J.Urol.83. 1469-1477 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohama, T.: "Neuroanatomical studies on pontine urine storage facilitatory areas in the cat brain. Part II.Output neural structure from the nucleus locus subcoeruleus and the nucleus reticularis pontis oralis." J.Urol.83. 1478-1483 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, K., Noto, H., Nishizawa, O., Sugaya, K., Suzuki, T., Shimoda, N., Otomo, R.and Tsuchida, S.: "Brainstem control of the internal and external urethral sphincter activity in decerebrate dog." J.Urol.147. 348A (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, K.: "Pontine control of the internal urethral sphincter activity on decerebrate dogs." Jap.J.Urol.84. 497-506 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, T.: "Alpha adrenergic mechanism on the reflex micturition in the male decerebrate dog." Jap.J.Urol.84. 711-719 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishizawa, O., Sugaya, K., Suzuki, T., Satoh, K., Kohama, T., Shimoda, N., Otomo, R., Harada, T., Tsuchida, S., Mori, S.: "Effect of YM-12617 (AMSULOSIN HYDROCHLORIDE) on lower urinary tract function in the female decerebrate dog." Acta Urol.Jan.38. 891-895 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noto, H., Fujieda, N., Sasaki, R., Kawahara, T., Shimoda, N., Nishizawa, O., Harada, T., Tsuchida, S.and Yamagishi, R.: "Effect of L-arginine and methylene blue on the micturition reflex in decerebrate dogs." Proc.23rd ICS. 23. 99 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, T., Nishizawa, O., Noto, H., Shimoda, N., Suzuki, K., Fujieda, N., Yamashiro, S., Harada, T.and Tsuchida, S.: "Effect of the inhibitors of nitric oxide/cyclic GMP pathway on the internal urethral relaxation in anesthetized rats." Proc.23rd ICS. 23. 102 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, N., Noto, H., Satoh, K., Nishizawa, O., Harada, T.and Tsuchida, S.: "Role of sacral alpha-adrenoceptive mechanisms in micturition reflex in the decerebrate dog." Proc.23rd ICS. 23. 108 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noto, H., Kawahara, T., Shimoda, N., Suzuki, T., Nishizawa, O., Harada, T.and Tsuchida, S.: "Internal urethral sphincter relaxation mediated by a non-adrenergic and non-cholinergic mechanism in decerebrate cats." J.Urol.149. 341A (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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