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1992 Fiscal Year Final Research Report Summary

Establishment of Analytical System for Metabolite Transport in Animal and Plant Organelles

Research Project

Project/Area Number 03559002
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionNagoya University

Principal Investigator

YAMAGUCHI Junji  Sch.Agr.SCI., Nagoya Univ.Assistant Professor, 農学部, 助手 (10183120)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Ryo  Sumitomo Chemical Co., Ltd.Research head, 主任研究員
WATANABE Takuji  Tomy Corporation Chief, 主任
UENO Yasuhiro  Bear Technology Inc.Director, 社長
KOJIMA Mineo  Sch.Agr.Sci., Nagoya Univ.Associate Professor, 農学部, 助教授 (30023469)
Project Period (FY) 1991 – 1992
KeywordsOrganelle / Hormone / Nuclei / Amyloplast / Chloroplast / Centrifugation
Research Abstract

(1) Separation and fractionation of intact organelles from animal and plant cells and
(2) establishment of precise and rapid analytical system for metabolite transport are final aiming in the project. As a first step, we attempted to make improved tissue homogenizer for aim (1) and to make new centrifugation system for aim (2). Using these systems, we succeeded in isolating nuclei, amyloplasts and chloroplasts from plant cells and confirmed the hypothesis that ADPglucose is directly transported into amyloplasts.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] J.Pozueta-Romero: "ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase." FEBS Lett.291. 233-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.YAMAGUCHI: "Isolation and characterization of nuclei form rice embryos." Cell Struc.Func.17. 87-92 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.-Y.Lim: "Distinct profiles of ADP-and UDP-specific sucrose synthases in developing rice grains." Biosci.Biotech.Biochem.56. 695-696 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.HATANO: "The preparation of high-molecular-weight DNA from rice and its analysis by pulsed-field gel electrophoresis." Plant Sci.83. 55-64 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Chevalier: "Nucleotide sequence of a cDNA for catalase from Arabidopsis italiana." Plant Physiol.99. 1726-1728 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Perata: "Artifactual detection of ADP dependent sucrose synthase from Nucleotide dephosphate activity." FEBS Lett.309. 283-287 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.YAMAGUCHI: "Isolation and characterization of nuclei form rice embryos." Cell Struc.Func.17. 87-92 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.HATANO: "The preparation of high-molecular-weight DNA from rice and its analysis by pulsed-field gel electrophoresis." Plant Sci.83. 55-64 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Saint-Guily: "cDNA sequence of an adenylate translocator from Arabidopsis thaliana." Plant Physiol.100. 1069-1071 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] P.Perata: "Effect of anoxia on starch breakdown in rice and wheat seeds." Planta. 188. 611-618 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.MITSUNAGA: "Production of α-amylase is repressed by uniconazole,an inhibitor of the biosynthesis of gibberellin,in a dwarf mutant of rice,Waito-C." Plant Cell Physiol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口 淳二: "種子貯蔵デンプン分解系." 植物細胞工学. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口 淳二: "酵素実験法(I)" 広川書店, 8 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口 淳二: "蛋白質・核酸・酵素(植物の分子生物学)" 共立出版, 10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Pozueta-Romero: "ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase." FEBS Lett.291. 233-237 (2991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.-Y.Lim: "Distinct profiles of ADP-and UDP-specific sucrose synthases in developing rice grains." Biosci. Biotech. Biochem.56. 695-696 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.Yamaguchi: "Isolation and characterization of nuclei form rice embryos." Cell Struc.Func.17. 87-92 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Hatano: "The preparation of high-molecular-weight DNA from rice and its analysis by pulsed-field gel electrophoresis." Plant Sci.83. 55-64 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.Perata: "Artifacutual detection of ADP dependent sucrose synthase from Nucleotide dephosphate activity." FEBS Lett.309. 283-287 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Chevalier: "Nucleotide sequence of a cDNA for catalase from Arabidopsis thaliana." Plant Physiol.99. 1726-1728 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] P.Perata: "Effect of anoxia on starch breakdown in rice and wheat seeds." Planta. 188. 611-618 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Saint-Guily: "cDNA sequence of an adenylate trans-locator from Arabidopsis thaliana." Plant physiol.100. 1069-1071 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mitsunaga: "Production of alpha-amylase is repressed by uniconazole, an inhibitor of the biosynthesis of gibberellin, in a dwarf mutant of rice, Waito-C." Plant Cell Physiol.(1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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