• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

A study on the temperature-dependent modes of selective transmission of mitochondria in Drosophila

Research Project

Project/Area Number 03640538
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionOchanomizu University

Principal Investigator

MATSUURA Etsuko T.  (Instructor) Dept. of Biology Ochanomizu University, 理学部, 助手 (00111691)

Co-Investigator(Kenkyū-buntansha) NIKI Yuzo  (Associate Professor) Dept. of Biology Ibaraki University, 理学部, 助教授 (00134164)
Project Period (FY) 1991 – 1992
KeywordsDrosophila / Mitochondrial transmission / Mitochondrial DNA / Heteroplasmy
Research Abstract

The temperature-dependent modes of selective transmission of mitochondrial DNA (mtDNA) were investigated in heteroplasmic lines which were established by germ-plasm transplantation in Drosophila melanogaster. The effects of the nuclear genome on the temperature- dependency were demonstrated in two sets of heteroplasmic lines: one established by reciprocal transplantation of germ plasm in D. melanogaster and the other derived from two D. melanogaster heteroplasmic lines possessing D. mauritiana mtDNA in which the nuclear genomes were reciprocally substituted. In each set, the temperature- dependent modes of selective transmission of the same two types of mtDNA were different depending on the nuclear genomes. Involvement of propagation of mitochondria and/or mtDNA replication in selective transmission was suggested from three observations, using heteroplasmic lines with appropriate combinations of two types of mtDNA and the nuclear genome. First, the intensity of selection was strongly affected by the temperature at the stage of oogenesis among the various developmental stages. Secondly, the intensity of selection was affected by some nucleotide sequences in the A+T-rich region containing the origin of replication. Thirdly, in heteroplasmic lines possessing two different sizes of mtDNA, the smaller one was selectively transmitted. In the last two cases,the effects of temperature were not clear. These results indicate that the nuclear genome is involved in determining the temperature-dependent mode of the selective transmission of mtDNA. They also suggest that the selective transmission is based on the differences in mtDNA replication. The present results will greatly contribute to clarify the molecular mechanisms of the regulation of mitochondrial transmission in eucaryotic cells.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] MATSUURA,E.T.,FUKUDA,H.and CHIGUSA,S.I.: "Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Reunion." Genetical research. 57. 123-126 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MATSUURA,E.T.,NIKI,Y.and CHIGUSA,S.I.: "Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster." Japanese Journal of Genetics. 66. 197-207 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] NAGATA,Y.and MATSUURA,E.T.: "Temperature-dependency od electron-transport activity in mitochondria with exogenous mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 255-261 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TSUJIMOTO,Y.,NIKI,Y.and MATSUURA,E.T.: "Further study on selective transmission of mitochondrial DNA in heteroplasmic lines of Drosophila melanogaster." Japanese Journal of Genetics. 66. 609-616 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MATSUURA,E.T.: "Selective transmission of mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 683-700 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KONDO,R.,MATSUURA,E.T.and CHIGUSA,S.I: "Further obseravation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. 59. 81-84 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura,E.T.: "Mitochondrial transmission in Drosophila.pp.213-221 in mechanisms of Molecular Evolution edited by N.Takahata and A.G.Clark" Japan Scientific Societies Press, Tokyo and Sinauer, Sunderland,U.S.A., 1993, 250 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuura, E. T., Fukuda, H. and Chigusa, S. I.: "Mitochondrial DNA heteroplasmy maintained in natural populations of Drosophila simulans in Reunion." Genetical Research. 57. 123-126 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura, E. T., Niki, Y. and Chigusa, S. I.: "Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster." Japanese Journal of Genetics. 66. 197-207 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, Y. and Matsuura, E. T.: "Temperature-dependency of electron-transport activity in mitochondria with exogenous mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 255-261 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsujimoto, Y., Niki, Y. and Matsuura, E. T.: "Further study on selective transmission of mitochondrial DNA in heteroplasmic lines of Drosophila melanogaster." Japanese Journal of Genetics. 66. 609-616 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura, E. T.: "Selective transmission of mitochondrial DNA in Drosophila." Japanese Journal of Genetics. 66. 683-700 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondo, R., Matsuura, E. T. and Chigusa, S. I.: "Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method." Genetical Research. 59. 81-84 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuura, E. T.: "Mitochondrial transmission in Drosophila." Mechanisms of Molecular Evolution, edited by N. Takahata and A. G. Clark. Japan Scientific Societies Press, Tokyo and Sinauer, Sunderland, U.S.A.213-221 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi