1992 Fiscal Year Final Research Report Summary
Genetics of Major Histocompotibility Complex
| Project/Area Number |
03640539
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
遺伝学
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| Research Institution | Dept. Biol., Facl. Sci., Nagoya Univ. (1992)
Hiroshima University (1991) |
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Principal Investigator |
HORI Hiroshi Nagoya Univ., Dept.Sci., Professor, 理学部, 教授 (60116663)
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| Project Period (FY) |
1991 – 1992
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| Keywords | MHC / Genetic Polymorphism / HLA / SSCP / Matching / Transplantation |
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| Research Abstract |
The MHC(Major Histocompatibility Complex, HLA in human) antigens are highly polymorphic glycoproteins expressed on the cell surface of all tissues (class I MHC) and antigen-presenting cells (class II MHC). These antigens play a key role in immune recognition of self and non-self antigens by T cells. Allologous MHC antigens are involved in graft rejection in tissue transplantation.
While MHC can be serologically defined in common clinical cases, recent advances in molecular biological techniques, especially the development of polymerase chain reaction (PCR), have facilitated more accurate analysis of the polymorphism in MHC at the DNA level. In this project, we have developed a rapid typing method based on the detection of single-stand conformation polymorphism, and formation of heteroduplexes in PCR products.
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[Publications] Hori,H., Satow,Y., Inoue,I., Chihara,M.: "Archaebacteria us Metabacteria: phylogenetic tree of Organisms indicated by comparison of 55 rDNA sequences" Evolution of Life (Osawa,S., Honjo,T. eds.) Springer, Tokyo/Berlin. 325-336 (1991)
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「研究成果報告書概要(和文)」より
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[Publications] Kimura,A.,Fukuda,Y.,Hori,H.,Hoshino,S.,Sasazuki,T.,Dohi,Y.: "Polymerase chain reaction -single-strand conformation polymorphism analysis of HLA-DP genes and its application in transplantation." Transplantation Proceedings. 25. 199-202 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Hoshino,S.,Kimura,A.,Fukuda,Y.,Hori,H.,Satow,,Y.,Sasazuki,T.,Dohi,Y.: "A simple and practical test of HLA-DRB and DQ-B matching in transplantation." Transplantation Proceedings. 25. 191-193 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Hori,H.,Satow,Y.,Inoue,I.,Chihara,M.: "Archaebacteria VS Metabacteria: Phylogenetic tree of organisms indicated by comparison of 5S ribosomal RNA sequences(Eds.: Osawa, S. and Honjo, T.), Springer, Berlin/Tokyo" Evolution of Life. 325-336 (1991)
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「研究成果報告書概要(欧文)」より
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