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1992 Fiscal Year Final Research Report Summary

Flux of organic matter from phytoplankton to bacteria

Research Project

Project/Area Number 03640552
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生態学
Research InstitutionNagoya University

Principal Investigator

HAMA Takeo  Nagoya University Water Research Institute Research associate, 水圏科学研究所, 助手 (30156385)

Project Period (FY) 1991 – 1992
KeywordsPhytoplankton / Bacteria / Microbial loop / Excretion / Fatty acids / ^<13>C-GC-MS method
Research Abstract

1.Estimation of ^<13>C isotopic ratio of fatty acid by gas chromatography-mass spectrometry(GC-MS): The accuracy of the estimation of ^<13>C isotopic ratio of fatty acid was ascertained by using ^<13>C enriched palmitic acid. The results indicated the applicability of ^<13>C-GC-MS method to estimate the carbon flow from phytoplankton to bacteria.
2.Production of fatty acid at subarctic and subtropical area of Northern Pacific: ^<13>C tracer experiments were carried out at subarctic and subtropical area of Northern Pacific. Fatty acid composition of photosynthetic products of phytoplankton was determined by ^<13>C-GC-MS method. Polyunsaturated fatty acids constituted major part of products at subarctic area, whereas saturated fatty acids were main product at subtropical region. This defference was mainly due to the difference in the water temperature of these region. High contribution of polyunsaturated fatty acids at subarctic region can keep the fluid condition of membrane of phytoplankton.
3.Carbon flux to the bacterial specific fatty acids: The ^<13>C atomic ratio of bacterial specific fatty acids were estimated by GC-MS to elucidate carbon flow from phytoplankton to bacteria. Samples obtained from subarctic and subtropical region were analyzed. The ^<13>C atom % of bacterial specific fatty acids were much lower than phytoplankton fatty acids, indication that carbon flow form phytoplankton to bacteria was not high which has been suggested by ^<14>C tracer studies. However, the effects of same factors such as incubation length and ^<13>C atom% of phytoplankton carbon must be considered to estimate carbon flux.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Hama,T.: "Production and turnover rates of fatty acids in marine particulate matter through phytoplankton photosynthesis" Marine Chemistry. 33. 213-227 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hama,T.: "Primary productivity and photosynthetic products around the Kuroshio warm-core ring" Deep-Sea Research. 39S1. S279-S293 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hama,T.: "Fatty acid composition in photosynthetic products of natural phytoplankton population in Lake Biwa,Japan" J.Plankton Research. 14. 1055-1065 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hama,T.: "13C tracer methodology in microbial ecology with special reference to primary production processes in aquatic enviroments" Advances in Microbial Ecology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hama: "Production and turnover rates of fatty acids in marine particulate matter through phytoplankton photosynthesis." Marine Chemistry. 33. 213-227 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hama: "Primary productivity and photosynthetic products around the Kuroshio warm-core ring." Deep-Sea Research. 39S1. S279-S293 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hama: "Fatty acid composition in photosynthetic products of natural phytoplankton population in Lake Biwa, Japan." J. Plankton Research. 14. 1055-1065 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hama: "^<13>C tracer methodology in microbial ecology with special reference to primary production processes in aquatic environments." Adv. Microbial Ecol.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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