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1993 Fiscal Year Final Research Report Summary

The yeast gene controlling over the initiation of sexual reproduction

Research Project

Project/Area Number 03640573
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

SHIMODA Chikashi  Osaka City University, Faculty of Science, Associate Professor, 理学部, 助教授 (80047290)

Project Period (FY) 1991 – 1993
KeywordsFission yeast / meiosis / sexual reproduction / RNA helicase / mating / specific antibody / DEAD protein / Drosophila
Research Abstract

The fission yeast ste13^+ gene which encodes a putative ATP-dependent RNA helicase is essential for initiation of sexual reproduction in response to nitrogen-starvation signal. The ste13^+ gene and its products were analyzed to verify our hypothesis that Ste13 regulates gene expression during sexual cycle at a post-transcriptional level and the following results were obtained.
(1)In vitro mutagenesis of ste13 :
The ste13 null mutant was defective in mating and meiosis but able to grow in rich medium. Site-directed mutagenesis introduced into the consensus motifs of RNA helicases caused the sterile phenotype. These facts indicate that the ste13 gene is not essential for mitotic cell division but indispensable for induction of sexual cycle.
(2)Intracellular localization of the ste13 protein :
The GST-ste13 fusion proteins were overproduced in E.coli. The affinity-purified fusion protein was used as the antigen to prepare a polyclonal antibody. Western blot analysis revealed that this antibody recognized a 58 kDa polypeptide in S.pombe cell extracts. Immunofluorescence microscopy with anti-ste13 showed that the ste13 protein was not localized in the nucleus and distributed in the cytoplasm.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] A.Hirata: "Structural modification of spindle pole bodies during meiosis II is essential for the normal formation of ascospores in Schizo-saccharomyces pombe:Ultrastructural analysis of spo mutants" YEAST. 10. 173-183 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Maekawa: "The ste13^+ gene encoding a putative RNA helicase is essential for nitrogen starvation-induced G1 arrest and initiation of sexual development in the fission yeast." Mol.Gen.Genet.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Aono: "Mating pheromone-induced expression of the mat1-Pm gene of Schizosaccharomomyces pombe:Identification of signalling componets and characterization of upstream controlling elements" YEAST. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kishida: "Meiosis-dependent mRNA splicing of the fission yeast Schizo-saccharomycespombe mes1+gene" Curr.Genet.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 北村憲二: "細胞周期とG1サイクリン" 蛋白質核酸酵素. 39. 170-175 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下田親: "分裂酵母の減数分裂遺伝子" 蛋白質核酸酵素. 39. 458-466 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maekawa, H.: "The ste13^+ gene encoding a putative RNA helicase is essential for nitrogen starvation-induced G1 arrest and initiation of sexual development in the fission yeast." Mo1.Gen.Genet. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aono, T: "Mating pheromone-induced expression of the mat1-pm gene of Schizosaccharomyces pombe : Identification of signalling components and characterisation of upstream controlling elements." Yeast. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kishida, M: "Meiosis-dependent mRNA splicing of the fission yeast Schizosaccharomyces pombe mesl^+ gane" Curr.Genet.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, A: "Structural modification of spindle pole bodies (SPBs) in meiosis II is essential for the normal ascospore formation in Schizosaccharomyces pombe : Ultrastructural analysis of spo mutants" Yeast. 10 :. 173-183 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, A: "Electron microscopic examination of sporulation-deficient mutants of the fission yeast Schizo-saccharomyces pombe" Arch.Microbiol.158 :. 249-255 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamura, K: "The novel gene trsl encodes an essential protein for the fransition from mitotic cell cycle to resting state in Schizosacchromyces pombe" FEMS Microbiol. Lett.84 :. 1-6 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kitamura, K: "The Schizosaccharomyces pombe mam2 gene encodes a putative pheromone receptor which has a significant homology with the Saccharomyces cerevisiae Ste2 protein." EMBO J.10 :. 3743-3751 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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