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1993 Fiscal Year Final Research Report Summary

cDNA cloning of enzymes involved in the synthesis and degradation of cell wall-matrix polysaccharides

Research Project

Project/Area Number 03640574
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

KAMISAKA Seiichiro  Osaka City Univ. Faculty of Science, Professor, 理学部, 教授 (60047214)

Co-Investigator(Kenkyū-buntansha) HOSON Takayuki  Osaka City Univ. Associate Professor, 理学部, 助教授 (70135771)
Project Period (FY) 1991 – 1993
KeywordsAuxin / Xyloglucan / Glucanase / Fucosidase / Arabinoxylan / Xyloglucanase / Feruloy1-CoA / Esterase
Research Abstract

The mechanical properties of cell walls are determined by cell wallconstituting molecules, particularly matrix polysaccharides. The goal of this study is to clone cDNA of enzymes involved in the synthesis and degradation of xyloglucans and arabinoxylans, which are responsible for determining cell wall extensibility. Results are as follows :
(1) Matrix polysaccharide-degrading enzymes : Xyloglucan degrading enzymes were solubilized with NaCl from Vigna epicotyl cell walls. The enzymes were fractionated and purified using Con A-Sepharose, Mono S, Superdex 75, and Phenyl Superose column. The enzymes are separated into 3 peaks on Mono S colum. alpha-Fucosidases which eliminate fucos-sede chain of xyloglucans were separated into Mono S-binding and -not binding fractions.
(2) Matrix polysaccharide-modifying enzymes : Using Golgi-vesicle fraction, the screening of the enzyme that ester-links ferulic acid to arabinose moieties of arabinoxylans. Oat arabinoxylans and synthesitic feruloyl-CoA were used as the substrates. Unfortunately, the enzyme activity was not detected, because of the high activity of feruloyl-CoA esterase in the enzyme preparation. At present, extensive effort has been done to eliminate the esterase from the enzyme preparation.
The present srudy will soon lead to the cloning of cDNA of the enzymes responsible for the synthesis and degradation of matrix polysaccharides determing the mechanical properteis of cell walls, although the cDNA cloning was not done within the research period of 3 years.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Tan,K.-S.: "Correlation between cell wall extensibility and the content of diferulic and ferulic acids in cell walls of Oryza sativa coleoptiles grown under water and in air." Physiol.Plant.83. 397-403 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tan,K.-S.: "Involvement of cell wall-bound diferulic acid in light-induced decrease in growth rate and cell wall extensibility of Oryza coleoptiles." Plant & Cell Physiology. 33. 103-108 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tan,K.-S.: "Effect of ferulic and p-coumaric acids on Oryza coleoptile growth and the mechanical properties of cell walls." J.Plant Physiology. 140. 460-465 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hoson,T.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl segments." Physiol.Plant.87. 142-147 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hoson,T.: "Regulation of polysaccharide breakdown during auxin-induced cell wall loosening." J.Plant Res.106. 369-381 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuda,Y.(編): "Plant Cell Walls as Biopolymers with Physiological Functions" Yamada Science Foundation, 452 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tan, K.-S et al.: "Correlation between cell wall extensibility and the content of diferulic and ferulic acids in cell walls of Oryza sativa coleoptiles grown under water and in air" Physiol.Plant.83. 397-403 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tan, K.-S.et al.: "Involvement of cell wall -bound diferulic acid in ligh-tinduced decrease in growth rate and cell wall extensibility of Oryza coleoptiles" Plant Cell Physiol.33. 103-108 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tan, K.-S.et al.: "Effect of ferulic and p-coumaric acids on Oryza coleoptile growth and the mechanical properties of cell walls" J.Plant Physiol.140. 460-465 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HOSON,T.et al.: "Role of xyloglucan breakdown in epidermal cell walls for auxin-induced elongation of azuki bean epicotyl segments" Physiol.Plant.87. 142-147 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hoson, T: "Regulation of polysaccharide breakdown during auxin-induced cell wall loosening" J.Plant Res. 106. 369-381 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuda, Y.(Ed.): Plant Cell Walls as Biopolymers with Physiological Functions. Yamada Science Foundation, (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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