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1992 Fiscal Year Final Research Report Summary

Dendritic Membrane Properties of Crayfish Nonspiking Interneurons

Research Project

Project/Area Number 03640592
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionHokkaido University

Principal Investigator

TAKAHATA Masakazu  Hokkaido University,Research Institute for Electronic Science,Associate Professor, 電子科学研究所, 助教授 (10111147)

Project Period (FY) 1991 – 1992
Keywordscrayfish / nonspiking interneuron / dendrite / outward rectification / single electrode voltage clamp / voltage-dependent outward current / delayed rectifier / synaptic integration
Research Abstract

Physiological properties of the dendritic membrane of LDS interneuron, an identified nonspiking interneuron in the terminal abdominal ganglion of the crayfish Procambarus clarkii Girard, have been studied using the single electrode voltage clamp (SEVC) technique. The site of electrode penetration into the dendrite was visualized under the dissecting microscope in situ during experiment by epiilluminating the Lucifer-filled cell and the microelectrode with blue light.
The dendritic membrane showed outward rectification to intracellularly injected current both at the thick branch on the midline and at the peripheral branches. Accordingly, the membrane time constant was shorter during depolarization than during hyperpolarization. The time course of compound PSP evoked by direct stimulation of presynaptic sensory neurons became shorter as the cell was more depolarized from the resting potential and vice versa. In contrast to the rectifying membrane property which was observed at any site of … More dendrites, the input resistance and membrane time constant were dependent of electrode penetration sites. Thus, the input resistance was significantly lower at the thick bridge portion than at peripheral branches.
A single intracellular microelectrode was placed at the bridge portion and the SEVC technique was applied to LDS dendrites. In response to hypepolarizing command voltage (10-50mV) applied to the cell held at the resting potential, a small inward leakage current was observed in proportion to the hyperpolarization. By contrast, the cell showed an additional outward current superimposed on the outward leakage current. The amount of additional current was dependent of the amount of depolarization. This current showed transient increase at the onset of depolarization and became steady in about 80-100msec. The finding that the steady outward current was reduced to 70-80% by perfusion of 10mM TEA indicates that it resembles the well known delayed rectifier K current. Characteristics of the transient early current are to be further studied. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] TAKAHATA,Masakazu: "Local nonspiking interneurons as separate integrator of motoneurons in crayfish" Comparative Biochemistry and Physilogy. 98. 13-18 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAKAHATA,Masakazu: "Feedforward excitation of telson flexor in-hibitors in the crayfish escape system" Neuroscience Research. Supl.14. S9- (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAKAHATA,Masakazu: "Multiple gate control of the descending stato-cyst-motor pathway in the crayfish Procambarusclarkii Girard" Journal Comparative Physiology. 170. 463-477 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahata,Masakazu: "Synaptic mechanisms underlying the interaction between postural reflexes and locomotor behavior in crayfish.In:"Neurobiological Basis of Human Locomotion"(ed.M.Shimamura et al.)pp.93-101" Japan Scientific Society Press/Tokyo, 447 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Takahata and M. Maki: "Multiple gate control of the descending statocyst-motor pathway in the crayfish Procambarus clarkii Girard" J. Comp. Physiol.170. 463-477 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takahata and M. Hisada: "Local nonspiking interneurons as a separate integrator of motoneurons in crayfish" Comp. Biochem. Physiol.98C. 13-18 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takahata: "Feedforward excitation of telson flexor inhibitors in the crayfish escape system" Neurosci. Res.Suppl.14:. S9 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Takahata: "Synaptic mechanisms underlying the interaction between postural reflexes and locomotor behavior in crayfish" "Neurobiological Basis of Human Locomotion" (ed. M. Shimamura, S. Grillner and V. R. Edgerton). Japan Scientific Society Press Tokyo. 93-101 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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