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1992 Fiscal Year Final Research Report Summary

Studies on the mechanism of flagellar motility.

Research Project

Project/Area Number 03640596
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionUniversity of Tokyo

Principal Investigator

SHINGYOJI Chikako  University of Tokyo.Faculty of Science,Assistant, 理学部, 助手 (80125997)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Keiichi  University of Tokyo.Faculty of Science,Emeritus Professor., 理学部, 名誉教授 (40011481)
Project Period (FY) 1991 – 1992
KeywordsFlagellar motility / sperm / microtubule / sliding velocity
Research Abstract

The movement of eukaryotic cilia and flagella is characterized by rhythmic generation of bending waves. There is ample evidence that mechanical and chemical conditions influence the frequency of the flagellar oscillation, however, no systematic investigation has been made so far on the effect of changing the beat frequency on other parameters of flagellar movement, because no reliable method has been available with which one can either increase or decrease the beat frequency reversibly.
Our introduction of imposed head vibration of a sperm (Gibbons et al., 1987) allowed us to manipulate the flagellar beat frequency reversibly. In the present study, the heads of demembranated spermatozoa of the sea urchin Tripneustes gratilla, reactivated at different concentrations of ATP, were held by suction in the tip of a micropipette and vibrated laterally with respect to the head axis. This imposed vibration resulted in a stable rhythmic beating of the reactivated flagella that was synchronized to … More the frequency of the micropipette. The reactivated flagella, which in the absence of imposed vibration had an average beat frequency of 39 Hz at 2 mmol 1^<-1> ATP, showed stable beating synchronized to the pipette vibration over a range of 20-70 Hz. At ATP concentrations of 10-100 mumol 1^<-1>, the range of vibration frequency capable of maintaining stable beating was diminished; increase in the ATP concentration above 2 mmol 1^<-1> had no effect on the range of stable beating. In flagella reactivated at ATP concentrations above 100 mumol 1^<-1>, the apparent time-averaged sliding velocity of axonemal microtubules decreased when the imposed frequency was below the unimposed flagellar beat frequency, but at higher imposed frequencies it remained constant, with the higher frequency being accompanied by a decrease in bend angle. This maximal sliding velocity at 2 mmol1^<-1> ATP was close to the sliding velocity in the distal region of live sperm, possibly indicating that it represents an inherent limit in the velocity of active sliding. The results are consistent with the view that the sliding velocity of axonemal microtubules does not depend solely upon the local concentration of ATP, but is also dependent upon the oscillatory mechanism associated with initiation of new flagellar bends. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Eshel,D.: "The phase of sperm flagellar beating is not conserved over a brief imposed interruption." Exp.Cell Res.202. 552-555 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHINGYOJI,C.: "Effect of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa." J.Exp.Biol.156. 63-80 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHINGYOJI,C.: "Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea-urchin sperm without twisting the axoneme." J.Cell Sci.98. 175-181 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAKAHASHI,K.: "Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea-urchin spermatozoa." J,Cell.Sci.98. 183-189 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eshel,D.: "Evidence for an inequality in the forces that generate principal and reverse bends in sperm flagella." J,Cell Sci.100. 213-218 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Eshel, D., C. Shingyoji, K. Yoshimura, I. R. Gibbons and K. Takahashi: "The phase of sperm flagellar beating is not conserved over a brief imposed interruption." Exp. Cell Res.202. 552-555 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingyoji, C., I. R. Gibbons, A. Murakami and K. Takahashi: "Effect of imposed head vibration on the stability and waveform of flagellar beating in sea urchin spermatozoa." J. Exp. Biol.156. 63-80 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shingyoji, C., J. Katada, K. Takahashi and I. R. Gibbons: "Rotating the plane of imposed vibration can rotate the plane of flagellar beating in sea-urchin sperm without twisting the axoneme." J. Cell Sci.98. 175-181 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, K., C. Shingyoji, J. Katada, D. Eshel and I. R. Gibbons: "Polarity in spontaneous unwinding after prior rotation of the flagellar beat plane in sea-urchin spermatozoa." J. Cell Sci.98. 183-189 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eshel, d., C. Shingyoji, K. Yoshimura, I. R. Gibbons and K. Takahashi: "Evidence for an inequality in the forces that generate principal and reverse bends in sperm flagella." J. Cell Sci.100. 213-218 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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