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1993 Fiscal Year Final Research Report Summary

Detection of Germ Cell Line in Marine Invertebrate

Research Project

Project/Area Number 03640604
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionOkayama University

Principal Investigator

YAMAMOTO Masamichi  Fac.Sci., Okayama Univ.Prof., 理学部, 教授 (50011565)

Co-Investigator(Kenkyū-buntansha) SHIRAI Hiroko  Fac.Sci., Okayama Univ.Assoc.Prof., 理学部, 助教授 (50090478)
Project Period (FY) 1991 – 1993
Keywordscephalopod / ascidian / starfish / germ cell / gonad / meiosis / ovary / testis
Research Abstract

1. The origin of the germ cell and the early processes of the gonad formation were clarified in a octopus, Octopus ocellatus. The earlygerm cells apear in the pericardial cavity. In the female, the germ cells begin meiosis just before hatching while in the females the germ cells enter into meiosis 7 weeks after hatching.
2. The origin of the germ cell and the process of sex differentiation of the gonads were elucidated in a solitary ascidian, Ciona intestinalis. The germ cells first appear in the juvenile just after metamorphosis in contact with the degenerating tissue mass which arises near the esophagus. The form a spherical body (early gonad), which divides into the testis and the ovary during migration along the wall of the stomach to the pyloric portion.
3. The origin of the germ cell and the process of gonad formation became clear in a star fish, Asterina pectinifera. The germ cells, about 10 in number, originate in the third coelomic pouch of the brachyolaria larvae. The enter into the haemal system and form the gonads after metamorphosis.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] M.A.B.Catalan: "The effect of pH on meiosis reinitiation in oocyte of the prosobranch mollusc,Ccttana nigrolineata." Invert.Repro.Dev.23. 211-213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of the prosobranch limpet,cellana nigrolineata." Invert.Repro.Dev.24. 127-136 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Inoue: "germ cell differentiation in starfish:The posterior entero coel as the origin of germ cells." Develop.Growth Differ. 34. 413-418 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kiyomoto: "Reconstruction of starfish eggs by electric fusion" Develop.Growth Differ.35. 107-114 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kiyomoto: "The determination for archenteron formation in starfish." Develop.Growth Differ.35. 99-105 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Takenaka: "Remodeling of the nauplius eye into adult oolli during metamorphosis of the barnacle." Develop.Growth Differ. 35. 245-255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Okada: "Identification of early oogenetic cells in the solitary ascidians." Develop.Growth Differ.35. 495-506 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Katayama: "phylogenetic position of acoel turbellarians inferred from partial 18S rDNA sequence" Zool.Sci.10. 536 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakairi: "Possible MIS production by follicle cells in spontaneous oocyte maturation of the ascidion." Develop.Growth Differ. 33. 155-162 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Inoue: "Origin of germ cells and early differentiation of gonads in starfish." Develop.Growth Differ. 33. 217-226 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of two Japanese species of Sipunculans:" Pacific Science. 48. 145-157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Sakairi & Shirai: "Possible MIS production by follicle cells in spontaneous oocyte maturation of the ascidian." Develop, Growth Differ.33. 155-162 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Inoue & H.Shirai: "Origin of germ cells and early differentiation of gonads in the starfish." Develop, Growth Differ.33. 217-226 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Inoue, M.Kiyomoto & H.Shirai: "Germ cell differentiation in starfish : The posterior enterocoel as the origin of germ cells." Develop, Growth Differ.34. 413-418 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Katayama, M.Yamamoto et al.: "Phylogenetic position of acoel turbellarians inferred from partial 18SrDNA sequence" Zool.Sci.10. 529-536 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okada & M.Yamamoto: "Identification of early oogenetic cells in the solitary ascidians Ciona savignyi and Ciona intestinalis." Develop.Growth Differ.35. 495-506 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Takenaka, M.Yamamoto et al.: "Remodeling of the naulpius eye into the adult ocelli during metamorphosis of the barnacle." Develop.Ggowth Differ.35. 245-255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kiyomoto & H.Shirai: "The determinant for archenteron formation in starfish : Co-culture of an animal egg fragment-derived call cluster" Develop.Growth Differ.35. 89-98 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kiyomoto & H.Shirai: "Reconstruction of starfish eggs by electric cell fusion : a new method to detect the cytoplasmic determinant." Develop.Growth Differ.35. 99-106 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Catalan & M.Yamamoto: "The effect of pH on meiosis reinitiation in oocytes of the prosobranch mollusc Cellana nigrolineata." Invert.Repro.Dev.23. 211-213 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Catalan & M.Yamamoto: "The annual reproductive cycle of the prosobranch limpet Cellana nigrolineata." Invert.Repro.Dev.24. 127-136 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Catalan & M.Yamamoto: "Annual reproductive cycle of two Japanese species of Sipunculans : Siphonosoma cumanense and Phascolosoma scolops." Pacific Sci.48. 145-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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