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1992 Fiscal Year Final Research Report Summary

Molecular Phylogeny of the Wild Populations of Medaka, Oryzias latipes

Research Project

Project/Area Number 03640637
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionThe Tokyo Metropolitan Institute of Medical Science

Principal Investigator

SAKAIZUMI Mitsuru  The Tokyo Metropolitan Institute of Medical Science, Department of Laboratory Animal Science, Researcher, 実験動物研究部門, 研究員 (40175360)

Project Period (FY) 1991 – 1992
KeywordsOryzias latipes / medaka / mitochondrial DNA / RFLP / sequence data / 12srDNA / cytochrome b / ND4
Research Abstract

Oryzias latipes includes four genetically distinct groups, the Northern population, the Southern population, the East Korean population and the china-West Korean population on the basis of electrophoretic data.
The Northern population and the Southern population were distinguished by RFLP (using eight 6-base enzymes) of mitochondrial DNA. The former included 10 haplotypes that were composed of a few groups closely related to each other. The latter included 89 haplotypes that could be divided into several groups with a lower level of difference. Sequence divergence was estimated to be 10.1% between a "northern" haplotype (haplotype 1) and a "southern" haplotype (haplotype 22).
This value was thought to be very high as those between intraspecific haplotypes.
We made two libraries of wkole mitochondrial DNA from a "northern" haplotype (1) and a "southern" haplotype (32). Three different regions encoding 12srRNA, cyt. b and ND4 were sequenced. The sequence differences between "northern" and " southern" haplotypes were 3.9% (15/586) in 12srDNA, 11.6% (64/554) in cyt. b and 9.9% (22/223) in ND4 region. Although the values differed among different regions, sequence divergence values were close to the estimate (10.1%) from RFLP analyses.
These results indicated that the Northern population and the Southern population are highly differentiated from a view point of molecular phylogeny.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] SAKAIZUMI M.: "Electrophoretic studies of meiotic segregation in inter-and intraspecific hybrids among East Asian species of thi genus Oryzias (Pices:Oryziatidae)." J.Exp.Zool.264. 85-92 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HAMAGUCHI S: "Sexually differentiated mechanisms of sterility in inter-specifichybrids between Oryzias latipes and O.curvinotus." J.Exp.Zool.263. 323-329 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KURITA J.: "Production of trigenomic allotriploid medaka Oryzias latipes sinensis-curvinotus-celebensis." Nippon Suisan Gakkaishi. 58. 6851-6855 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KURITA J.: "Productionof amphidiploid medaka Oryzias 2 latipes sinensis-2 curvinotus by gyno-genesis wirh retention of the second polar body." Nippon Suisan Gakkaishi. 59. 373 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAKAIZUMI M.: "Unreduced diploid eggs produced by interspecific hybrids between Oryzias latipes and O.curvinotus." J.Exp.Zool.IN PRESS

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今井 喜美子: "ミトコンドリアDNAからみたメダカの系統." 千葉大海洋センター年報. 12. 69-74 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamaguchi,Satoshi: "Sexually differentiated mechanisms of sterility in interspecific hybrids between Oryzias latipes and O. curvinotus" J.Exp.Zool.263. 323-329 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaizumi,Miotsur: "Electrophoretic studies of meiotic segregation in inter- and intraspecific hybrids among East Asian species of the genus Oryzias (Pisces : Oryziatidae)" J.Exp.Zool.264. 85-92 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurita,Jun: "Production of trigenomic allotriploid medaka Oryzias latipes sinensis- curvinotus- celebensis" Nippon Suisan Gakkaishi. 58. 2311-2314 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imai,Kimiko: "Mitochondrial DNA polymorphisms in wild populations of the medaka, Oryzias latip (In Japanese)" Ann.Rep.Marine Ecosys.Res Center, Chiba Univ.12. 69-74 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurita,Jun: "Production of amphidiploid medaka Oryzias 2 latipes sinensis-2 curvinotus by gyno- genesis with retention of the second polar body" Nippon Suisan Gakkaishi. 59. 373 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaizumi,M., Shimizu,Y., Matsuzaki,T., and Hamaguchi,S.: "Unreduced diploid eggs produced by interspecific hybrids between Oryzias latipes and O. curvinotus" J.Exp.Zool.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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