1992 Fiscal Year Final Research Report Summary
Cellular Biological Analysis of Secretion Mechanism under Nutrient Stress.
| Project/Area Number |
03660065
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NISHIZAWA Naoko The Univ. of Tokyo Agriculture assistant, 農学部, 助手 (70156066)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Satoshi The Univ. of Tokyo, Agriculture associate professor, 農学部, 助教授 (90011915)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Mugineic Acid / Nicotianamine / Iron-deficiency stress / Barley |
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| Research Abstract |
Fe-deficiency induces the biosynthesis of mugineic acid family phytosiderophores (MAs) in graminaceous plants. Using a cell free system derived from root tips of iron deficient barley, we clarified the biosynthetic pathway of deoxymugineic acid (DMA) from methionine in vitro [ Methionine S-adenosyl methionine Nicotianamine(NA) (3"-ketoform) DMA].
In order to isolate the genes encoding the biosynthetic enzymes of MAs, we tried to purify the enzymes which synthesize nicotianamine from SAM (NA synthase) and unidentified 3"-ketoform from NA. They were partially purified by using hydrophobic chromatography, gel filtration, ion exchange, and native electrophoresis.
The characteristics of both enzymes clarified until now are as fallows.
1. Biosynthetic activity of both processes were markedly induced by iron deficiency stress.
2. Both enzymes show the same optimum PH (9).
3. Molecular weight of NA synthase is 40-50KD.
4. The enzyme synthesizing 3"-ketoform needs 2-oxoglutarate and pyridoxal phosphate (PLP) as a cofactor, and its molecular weight is about 80KD.
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[Publications] Singh, K., Chino, M., Nishizawa, N. K., Goto, T., Nakanishi, T., Takagi, S., Mori, S.: "Iron extraction efficiency of plant borne mugineic acid family phytosiderophores in indian calcarious soils." J. of Plant Nutrition. 15. 1625-1645 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Shojima, S., Nishizawa, N. K., Fushiya, S., Nozoe, S., Takagi, S., Mori, S., Chino, M.: "Cross-reactivities of Anti-nicotianamine antibody with mugineic acid and Related compounds." Soil Sci. Plant Nutr.37. 159-162 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakanishi, H., Okumura, N., Umehara, Y., Nishizawa N. K., Chino, M, Mori, S.: "Expression of an Iron-deficiency specific clone (Ids 3) in roots of Hordeum vulgare." Plant & Cell Physiol.34.
Description
「研究成果報告書概要(欧文)」より
