1992 Fiscal Year Final Research Report Summary
Characterization of structural and regulatory genes of rice nitrite reductase.
| Project/Area Number |
03660084
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IDA Shoji Research Institute for Food Science Associate Professor, 食糧科学研究所, 助教授 (70027184)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Rice / Oryza sativa L / Nitrite reductase / cDNA library / cDNA sequence / cDNA with an intervening sequence |
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| Research Abstract |
Leaf and root cDNA libraries were constructed in lambdagt 11 phage vector from nitrate-induced greening rice seedlings. Two different types of nitrite reductase (NiR) cDNA from each library were cloned and sequenced. DNA sequence of one group had an open reading frame matched with the isolated NiR protein sequence, while cDNA clone from the other had an addtional sequence of 93 bp in the reading frame of the former clone. The insertion sequence has consensus sequence of 5' and 3' splice junction in eukaryotic mRNA. This inserted DNA sequence was confirmed to exist in rice nuclear genomic DNA by PCR technique using the genomic DNA as a template. Complete sequencing of the rice NiR gene may provide a clue to elucidating physiological significance of this unusual presence of an intervening sequence in NiR cDNA.
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