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1992 Fiscal Year Final Research Report Summary

Mechanisms for signal transduction in the initiation of meiosis in enkaryotes

Research Project

Project/Area Number 03660115
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YAMASHITA Ichiro  Center for Gene Science Hiroshima Univ. Assoc. Prof., 遺伝子実験施設, 助教授 (20144884)

Project Period (FY) 1991 – 1992
KeywordsMeiosis / Sporulation / Transcriptional Regulation / Protein Kinase
Research Abstract

1. We isolated IME2, SME2, and SME3 genes as positive regulators for the initiation of meiosis. IME2 was transcribed specifically at an early stage of meiosis. Transcripts from SME2 and SME3 were accumulated in stationary cells. SME2 activated transcription of SGA1, a late meiosis-specific gene, independently of IME1 and IME2. SME3 activated IME1 transcription. GAM1 and GAM3, positive regulators for STA1, were also required for transcription of IME1. SUD1, GAM2, NIM1, and NIM2 were required for repression of IME2. From these results, we proposed a model for regulatory cascades governing the initiation of meiosis. 2. Using IME2-specific antisera, we demonstrated nuclear localization and protein kinase activity of IME2 protein. Carboxy-terminal domain of IME2 consisting of six highly acidic subpeptides appeared to have a negative role. 3. We also examined the mechanism for transcriptional regulation of SGA1 by IME2 kinase. 5' upstream region of SGA1 contained positive (UAS) and negative (NRE) elements for transcription. UAS activity was not regulated by IME2. Deletion of NRE activated transcription of SGA1 independently of IME2. These results suggest that IME2 kinase relieves negative regulation through NRE. We also identified UAS- and NRE-binding proteins by gel shift assay.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Kihara: "Positive and negative elements upstreams of the meiosis-specific glucoamylase gene in Saccharomyces cerevisiae" Molecular and General Genetics. 226. 383-392 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yoshimoto: "The GAM1/SNF2 gene of Saccharomyces cerevisiae encodes a highly charged nuclear protein required for transcription of the STA1 gene" Molecular and General Genetics. 228. 270-280 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kawaguchi: "Nutritional regulation of meiosis-specific gene expression in Saccharomyces cerevisiae" Bioscience, Biotechnology, and Biochemistry. 56. 289-297 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yoshimoto: "The Saccharomyces cerevisiae GAM2/SIN3 protein plays a role in both activation and repression of transcription" Molecular and General Genetics. 233. 327-330 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yoshimoto: "Identity of the GAM3 gene with ADR6, each required for transcription of the STA1 or ADH2 gene in Saccharomyces cerevisiae" Bioscience, Biotechnology, Biochemistry. 56. 527-529 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山下 一郎: "清酒酵母の研究-'80年代の研究-" 清酒酵母研究会, 225 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Kihara: "Positive and negative elements upstream of the meiosis-specific glucoamylase gene in Saccharo-myces cerevisiae" Mol. Gen. Genet. 226. 383-392 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Yoshimoto: "The GAM1/SNF2 gene of Saccharomyces cerevisiae encodes a highly charged nuclear protein required for transcription of the STA1 gene" Mol. Gen. Genet. 228. 270-280 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Kawaguchi: "Nutritional regulation of meiosis-specific gene expression in Saccharomyces cerevisiae" Biosci. Biotech. Biochem. 56. 289-297 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Yoshimoto: "The Saccharomyces cerevisiae GAM2/SIN3 protein plays a role in both activation and repression of transcription" Mol. Gen. Genet. 233. 327-330 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Yoshimoto: "Identity of the GAM3 gene with ADR6, each required for transcription of the STA1 or ADH2 gene in Saccharomyces cerevisiae" Biosci. Biotech. Biochem. 56. 527-529 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I. Yamashita: The Association for Sake yeasts. Studies on Sake yeasts, 225 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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