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1993 Fiscal Year Final Research Report Summary

STUDIES ON THE CLEANUP METHOD OF ORGANO CHLORINATED COMPOUND CONTAMINATED SOILS BY MICROORGANISMS

Research Project

Project/Area Number 03660121
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionNATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES

Principal Investigator

YAGI Osami  NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES WATER AND SOIL ENVIRONMENT DIVISION SECTION CHIEF, 水土壌圏環境部, 室長 (40132865)

Co-Investigator(Kenkyū-buntansha) IWASAKI Kazuhiro  ibid.RESEARCHER, 地域環境研究グループ, 研究員 (30193717)
TOMIOKA Noriko  ibid.RESEARCHER, 水土壌圏環境部, 研究員 (40168399)
UCHIYAMA Hiroo  ibid.CHIEF RESEARCHER, 水土壌圏環境部, 主任研究員 (00185042)
Project Period (FY) 1991 – 1993
KeywordsVOLATILE ORGANOCHLORINATED COMPOUNDS / SOIL CLEANUP / GROUNDWATER POLLUTION / TRICHLOROETHYLENE / TETRACHLOROETHYLENE
Research Abstract

A methane-utilizing bacterium, Methylocystis sp. M (strain M) was isolated from soil. This strain degrades trichloroethylene(TCE). Strain M can degrade TCE at a relatively high concentration fo 35 mg/L and as well as various halogenated aliphatic compounds. Bioremediation of TCE-contaminated soil by strain M was investigated in soil microcosms, using 70 ml of serum bottles and 450-ml glass columns as the soil microcosms. Strain M was added to the microcosms filled with a TCE-contaminated soil and water mixture with TCE concentration ranging from 0.1 to 1 ppm. More than 95% of TCE of 0.1 ppm was degraded by the addition of strain M, and degradation of 1,1-, cis- and trans-dichloroethylene (DCE) was accelerated by the strain. H_2O_2 addition with strain M significantly promoted TCE degradation. Strain M proved to be very effective to cleaning up the TCE-contaminated soil.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] O.Yagi,H.Uchiyama,and K.Iwasaki: "Biodegradation Rate of Chloroethylene in Soil Environment" Wat.Sci.Tech.25. 419-424 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢木修身、内山裕夫: "揮発性有機塩素化合物の微生物による分解・除去" 水環境学会誌. 15. 493-498 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Uchiyama,T.Nakajima,O.Yagi,and T.Nakahara: "Role of Heterotrophic Bacteria in Complete Mineralization of Trichloroethylene by Methlocystis sp.strain M" Applied and Environmental Microbiology. 58. 3067-3071 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Nakajima,H.Uchiyama,O.Yagi,and T.Nakahara: "Novel Metabolite of Trichloroethylene in a Methanotrophic Bacterium,Methylocystis sp.M,and Hypothetical Degradation Pathway" Biosci.Biotech.Biochem.56. 486-489 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成瀬洋児、渡辺正敏、土山秀樹、矢木修身: "トリクロロエチレンの土壌への収・脱着" 水環境学会誌. 16. 346-353 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢木修身、内山裕夫: "バイオレメディエーション技術を用いる揮発性有機塩素化合物汚染土壌・地下水の浄化" BIO INDUSTRY. 10. 477-482 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷吉樹、矢木修身他: "バイオコンバージョン" 医学出版センター, 250 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松井三郎、矢木修身他: "環境微生物工学研究法" 技報堂, 450 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakajima, T., Uchiyama, H., Yafi, O.and Nakahara, T.: "Novel metabolite of trichloroethylene in a methanotrophic bactreium, Methylocystis sp.M, and hypothetical degradation pathway" Biosci.Biotech.Biochem.56. 486-489 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama, H., Nakajima, T., Yagi, O.and Nakahara, T.: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp.M" Appl.Environ.Microbiol.58. 3067-3071 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yagi, O., Uchiyama, H.and Iwasaki, K.: "Biodegradation rate of chloroethylene in soil environment" Wat.Sci.Tech.25. 419-424 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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