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1992 Fiscal Year Final Research Report Summary

Studies on initial tissue culture techniques of mangrove tree species in Okinawa, Japan

Research Project

Project/Area Number 03660160
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 林学
Research InstitutionUniversity of the Ryukyus

Principal Investigator

BABA Shigeyuki  Univ. of the Ryukyus, College of Agric. Assistant, 農学部, 助手 (30117585)

Co-Investigator(Kenkyū-buntansha) KINJO Kazuhiko  Univ. of the Ryukyus, College of Agric. Assistant, 農学部, 助手 (10167380)
SATO Kazuhiro  Univ. of the Ryukyus, College of Agric. Associate Prof., 農学部, 助教授 (50045126)
Project Period (FY) 1991 – 1992
Keywordsmangroves / Kandelia candel / Bruguiera gymnorrhiza / Rhizophora stylosa / tissue cultures / viviparous seed / callus induction
Research Abstract

In this study, attempts have been made to develop initial tissue culture techniques of several mangrove tree species distributed in Okinawa, Japan. The samples were collected from trees of Bruguiera gymnorrhiza, Kandelia candel, Rhizophora stylosa (Rhizophoraceae) and Hibiscus tiliaceus (Malvaceae) in Okinawa island, and trees of Avicennia marina (Verbenaceae) and Sonneratia alba (Sonneratiaceae) in lriomote island. Materials used for inducing calli were viviparous seeds of B. gymnorrhiza, K. candel and R. stylosa, semi-viviparous seeds of A. marina, and seeds of H. tiliaceus and S. alba. In the callus culture, viviparous seeds were cut in disc shaped particles in 5 mm thickness, then put on the media. Two regents of phytohormones, Kinetin as a cytokinin and 2, 4-D (2, 4-dichlorophenoxyacetic acid) as a auxin, were added for inducing calli to MS medium (Murashige and Skoog medium, 1962). Few contaminations were observed for using a modified sterilisation method combined with the ethyl alcohol and sodium hypochlorite solutions method.
There was no significant difference in the rates of callus inductions between any growing stages of viviparous seeds of B. gymnorrhiza, K. candel and R. stylosa when both kinetin and 2, 4-D were added to the callus inducing media. Calli were induced from hypocotyls of non-symbiotic seedlings of H. tiliaceus. The subcultures were done at the earlier stages of inducted calli of viviparous seeds, because those calli at the later stages became harder, and not grew well on the subculture media.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 馬場 繁幸: "オヒルギ、メヒルギの組織培養に関する基礎的研究" 琉大農学報.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬場 繁幸: "沖縄産マングローブ樹種の組織培養に関する基礎的研究(I)" 南資研研究会誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬場 繁幸: "沖縄産マングローブ樹種の組織培養に関する基礎的研究(II)" 日林九支研論集.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeyuki BABA: "Studies on initial tissue culture techniques of Bruguiera gymnorrhiza and Kandelia candel, Rhizophoraceae." Sci. Bull. Coll. Agric. Univ. Ryukyus.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeyuki BABA: "Studies on initial tissue culture techniques of mangrove tree species in Okinawa, Japan (I)." J. Soc. Trop. Res. Technol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeyuki BABA: "Studies on initial tissue culture techniques of mangrove tree species in Okinawa, Japan (II)." Trans. Mtg. Kyushu Branch J. For. Soc.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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