1992 Fiscal Year Final Research Report Summary

Study on Winter Sleepnized Preservation of Seawater-Acclimatized Chlorella with Condensed Cells

Research Project

Project/Area Number	03660201
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General fisheries
Research Institution	Tokai University
Principal Investigator	INOUE Motoo Department of Fisheries, School of Marine Science and Technology, Tokai University, 海洋学部水産学科, 教授 (00055667)
Project Period (FY)	1991 – 1992
Keywords	Chlorella / Seawater-Acclimatized Chlorella / Condensed Chlorella / High temperature strain / Dormancy / Winter sleep / Preservation / Living dry chlorella
Research Abstract	The object this study is to preserve sea-water acclimatized chlorella with condensation of 1 x 10^8 cells/ml as living chlorella. after winter sleepnized treatment to change mushy, gelatlniform and dried powdered conditions. As speclmens in the experiments, optimal temperature 35ﾟC of high temperature straln(Chlorella sp)and optimal temperature 25ﾟC of middle temperature strain were used. During the treatment for dormancy, the culture temperature was gradually decreased to at 5ﾟC and 0ﾟC for 1 week. Obtained results were follows. (1)In the treatment for dormancy, optimal temperatures for high temperature strain and middle temperature strain were 5ﾟC and 0ﾟC respectively. Among preservations for 1 month of musy, gelatiniform and dried powdered conditions under salinity of 50%, 35% and 70%, those chlorella under the treatment for dormancy showed good propagation atter their restoration.

(2 results)