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1992 Fiscal Year Final Research Report Summary

Metabolism of the lipoprotein secreted by eel hepatocytes cultured primarily.

Research Project

Project/Area Number 03660215
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionKagoshima University

Principal Investigator

HAYASHI Seiichi  Kagoshima University, Factulty of Fisheries Professor, 水産学部, 教授 (80041721)

Project Period (FY) 1991 – 1992
KeywordsEel / Lipoproteins / Primary culture of hepatocytes / Insulin / Lipoprotein lipase / Protein synthesis and secretion / Liposome / Muscle
Research Abstract

1. Cultured eel-hepatocytes synthesized and secreted the only one kind of lipoprotein, namely VLDL. Although there are two main lipoproteins in eel serum, VLDL and HDL, eel-hepatocytes did not synthesize and secrete HDL. 2. Characterizations of the VLDL. (1) Chemical composition of the VLDL. The contents of rotein, triacylglycerol, free cholesterol, cholesterol ester and phospholipid in the VLDL were 12, 69, 3.6, 0.4 and 14%, respectively. (2) Apoproteins of the VLDL. Main apoproteins of the VLDL were apo A and B. What is most different from VLDLs secreted by mammalian livers is that the secreted VLDLs of mammals do not contain apo A as a main apoprotein. (3) The rate of the VLDL secretion.
Cultured eel-hepatocytes secreted the VLDL at the rate of 3.79*1.51mug VLDL/mg cell protein/h(n=6). This value was 10 times higher than the rates of VLDL secretion by human hepatocytes. 3. Effects of insulin and serum lipoproteins. (1) Effect of insulin. Insulin inhibited the secretion of the VLDL and the incorporation of 14C-leucine and 14C-acetate into the VLDL. (2) Effect of serum VLDL. When serum VLDL was added to the eel-hepatocytes, the hepatocytes stimulated the VLDL secretion. Furthermore, the synthesis of apoproteins of the VLDL, especially apo B, was stimulated by the serum VLDL. 4. When the secreted VLDL was incubated with liposomes or lipoprotein lipase, a HDL-like particle was formed. 5. The presence of high content of lipid in eel muscle was histochemically observed. When the incorporation of 14C-VLDL into the muscle, liver, heart and kidney was investigated after the 14C-VLDL was injected into the portal vein, the incorporation into the muscle was the highest. Furthermore, it was found that there was the protein on the plasma membrane of eel muscle which could bind the VLDL specifically and of which molecular weight was 77,000.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Yu F.-G.: "Characterization of lipoprotein secreted by cultured eel hepatocytes and its cmoparison with serum lipoproteins." Cell Struct.Funct.16. 347-355 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu F.-G.: "Effects of insulin,eel serum very low density lipoprotein,and high density lipoprotein on lipoprotein syntheisis and secretion by cultured hepatocytes of eel(Anguilla japonica)." Comp.Biochem.Physiol.101B. 333-339 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu F.-G.: "Conversion of the lipoprotein secreted by cultured eel hepatocytes to high density lipoprotein." Comp.Biochem.Physiol.103B. 321-327 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi S.: "Lipoprotein synthesis and secretion by cultured eel hepatocytes.(Mini Review)" Comp.Biochem.Physiol.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 林 征一: "ラボマニュアルマリンバイオテクノロジー宮地 重遠監修 嵯峨 直恒 松永 是編集" 裳華房, 285 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu F. -G., Ando S. and Hayashi S.: "Characterization of lipoprotein secreted by cultured eel hepatocytes and its comaprison with serum lipoproteins." Cell Struct. Funct. 16. 347-355 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu F. -G., Ando S. and Hayashi S.: "Effects of insulin, eel serum very low density lipoprotein, and high density lipoprotein on lipoprotein synthesis and secretion by cultured hepatocytes of eel (Anguilla japonica)." Comp. Biochem. Physiol. 101B. 333-339 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu F. -G., Ando S. and Hayashi S.: "Conversion of the lipoprotein secreted by cultured eel hepatocytes to high density lipoprotein." Comp. Biochem. Physiol.103B. 321-327 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi S. and: "Lipoprotein synthesis and secretion by cultured eel hepatocytes. (Mini Review)" Comp. Biochem. Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi S.: Syokabou, Tokyo. Primary culture of eel and carp hepatocytes. in "Labo-manual of Marine Biotechnology" (in Japanese), 44-57 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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