1992 Fiscal Year Final Research Report Summary
Gene mapping with DNA markers in Japanese quail
| Project/Area Number |
03660277
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産学(含草地学)
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| Research Institution | Gifu University |
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Principal Investigator |
ITO Shin'ichi Gifu University, Faculty of Agriculture, Associate Professo, 農学部, 助教授 (20108062)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tomomasa Aichi Prefecture Colony, Institute for Developmental Research, Head, 発達障害研究所・生化学部, 室長 (10100174)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Japanese quail / DNA markers / RFLP / Restriction enzyme / Genetic markers / Linkage / Gene mapping / Genetic linkage map |
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| Research Abstract |
Restriction fragment length variations (RFLVs) were investigated for increasing genetic markers for drawing autosomal linkage maps in Japanese quail (Coturnix japonica). The best tissue for extracting genomic DNA from this species was lung in adults and brain in newly hatched chicks, respectively. For digesting DNA at the optimum temperature of 37ﾟC, the following 12 low-priced restriction enzymes were selected: Apa I, EcoR I, Pst I, BamH I, Hind I, Kpn I, EcoR I, Dra I, Pvu II, Xba I, Bgl II, Sac I. As probes, many random genomic clones were produced from the liver extract of a quail. Out of them, 12 probes were selected and were numbered consecutively for use. Six families composed of parents and their offspring with the information on sex and 17 autosomal genetic marker loci (seven plumage color loci, two eggshell color loci, and eight biochemical loci) were selected, Two sex-linked probes were detected. These are probes No.1 and No.3, which detected RFLVs in DNA digested with Pst I and EcoR I, respectively. From two families, these probes were clarified to be on Z chromosome. These probes will be useful for expanding genetic map on Z chromosome in Japanese quail and for studying the homology of Z chromosome in the chicken belonging the same family. No autosomal probes could not be detected from the above six families in spite of the use of many restriction enzymes. In future, more families will be investigated for detecting autosomal probes. No evidence of autosomal close linkage was detected among the 27 pairwise combinations including seven plumage color and six biochemical loci. Two new mutations were detected. One is named "celadon", which is an autosomal recessive eggshell color mutation. The other is called "light down", that is an autosomal dominant plumage color mutation with homozygous lethality. These mutations will be useful as new genetic markers for expanding linkage maps.
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