1992 Fiscal Year Final Research Report Summary
Genetic analysis of swine pneumonic mycoplasmas by PCR.
| Project/Area Number |
03660305
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | University of Tokyo |
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Principal Investigator |
HARASAWA Ryo University of Tokyo, Faculty of Medicine, Associate Professor, 医学部(医), 助教授 (70159101)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Mycoplasma / PCR / Swine enzootic pneumonia |
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| Research Abstract |
This study is focused on genetic analysis of Mycoplasma hyopneumoniae which is considered as an etiologic agent of the enzootic pneumonia in swine. The 16S-23S rRNA intergenic spacer regions of M. hyopneumoniae strain VPPll was amplified by PCR and sequenced by dideoxy chain termination method. The 16S-23S spacer region revealed following features: (1) lack of tRNA genes, (2) existence of "boxA"-like sequence, (3) promotor-like structure, (4) nested-palindromic sequences, (5) antitermonator structure.
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