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1992 Fiscal Year Final Research Report Summary

Genetic analysis of swine pneumonic mycoplasmas by PCR.

Research Project

Project/Area Number 03660305
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionUniversity of Tokyo

Principal Investigator

HARASAWA Ryo  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部(医), 助教授 (70159101)

Project Period (FY) 1991 – 1992
KeywordsMycoplasma / PCR / Swine enzootic pneumonia
Research Abstract

This study is focused on genetic analysis of Mycoplasma hyopneumoniae which is considered as an etiologic agent of the enzootic pneumonia in swine. The 16S-23S rRNA intergenic spacer regions of M. hyopneumoniae strain VPPll was amplified by PCR and sequenced by dideoxy chain termination method. The 16S-23S spacer region revealed following features: (1) lack of tRNA genes, (2) existence of "boxA"-like sequence, (3) promotor-like structure, (4) nested-palindromic sequences, (5) antitermonator structure.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] HARASAWA,R.,et al.: ""boxA"-like sequence between the 16s/23s spacer in rRNA operon of mycoplasmas." FEBS Letters. 297. 209-211 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] UEMORI,T.,et al.他3名: "Amphitication of the 16s-23s spacer region in rRNA operon of mycoplasmas by the polymerase chain reaction." Systematic and Applied Microbiology. 15. 181-186 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HARASAWA,R.et al 他4名: "Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis." Microbiology and Immunology. 36. 661-665 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] IMADA,Y.et al 他3名: "Sodium dodecyl sulfate-polyacrylamide gel electrophoreses and western bltting analyses of ureaplasmas isolated from dogs." Journal of Veterinary Sciences. 54. 325-328 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harasawa, R. et al.: ""boxA"-like sequence between the 16S/23S spacer in rRNA operon of mycoplasmas." FEBS Letters. 297. 209-211 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uemori, T., et al.: "Amplification of the 16S-23S spacer region in rRNA operon of mycoplasmas by the polymerase chain reaction." Systematic and Applied Microbiology. 15. 181-186 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harasawa, R. et al.: "Nucleotide sequence of the arginine deiminase gene of Mycoplasma homins." Microbiology and Immunology. 36. 661-665 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imada, Y. et al.: "Sodium dodecyl sulfate-poly-acrylamide gel electrophoresis and western blotting analyses of ureaplasmas isolated from dogs." Journal of Veterinary Sciences. 54. 325-328 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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