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1992 Fiscal Year Final Research Report Summary

NBT REDUCTION TEST, VITAMIN A・E AND MASTITIS

Research Project

Project/Area Number 03660325
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionKAGOSHIMA UNIVERSITY

Principal Investigator

HAMANA Katsumi  PROFESSOR, DEPARTMENT OF VETERINARY MEDICINE, FACULTY OF AGRICULTURE, KAGOSHIMA UNIVERSITY, 農学部, 教授 (30011977)

Co-Investigator(Kenkyū-buntansha) KAMIMURA Shunichi  ASSISTANT PROFESSOR, DEPARTMENT OF VETERINARY MEDICINE, FACULTY OF AGRICULTURE,, 農学部, 講師 (90233949)
Project Period (FY) 1991 – 1992
KeywordsDairy Cattle / NBT Reduction Test, / Vitamin A / Vitamin E / Mastitis, / Teat / Ultrasonography
Research Abstract

In this research, effects of vitamin A (Retinol) and vitamin E (alphatocopherol) on subclinical mastitis and on bactericidal ability of neutrophils were investigated. In addition, teats which is the most important factor in mastitis were investigated morphologically.
Forty dairy cattle kept in a good feeding and management farm were monthly examined on milk yield and quality, mastitis tests, blood analysis, serum vitamin A and E level for one year. Electronic conductivity and somatic cell counts in milk were found to have a relatively well correlation to milk yield and quality. Serum vitamin E levels was found to have some relation to these test results.
Effect of retinol and alpha-tocopherol was investigated in vitro on the bactericidal ability of neutrophils obtained from 20 lactating cows. Nitroblue tetrazolium (NBT) reduction test was employed. After the isolation of neutrophils from blood, NBT reduction test was conducted under the condition of intact, retinol and alpha-tocopherol a … More ddition. The most high NBT reduction ability was obtained at 100 IU/dl of retinol and at 200 mug/dl of alpha-tocopherol, respectivily. Ethanol, retinol or alpha-tocopherol were added to the medium of neutrophil's culture. Though NBT reduction ability decreased to about half level in control, it was well preserved in retinol or alpha-tocopherol addition. As a result, it was suggested that the bactericidal ability of neutrophils from lactating cows was influenced in vitro by the level of retinol or alpha-tocopherol, and was well preserved under the presence of retinol or alpha-tocopherol.
Several parts of the teats were measured and its results were compared to milk quality and bacteria. It was found that the teat with bigger teat gradient or teat canal diameter is liable to mastitis. Then, the teats were examined by ultrasonography, macroscopically and histopathologically. Abnormal findings were diagnosed by ultrasonography as follows, obstraction of teat sinus, hypertrophy of intima, abscess, polyp, diverticulum, stenosis and malformation. They were confirmed by morphological examination. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 浜名 克己,工藤 幸裕 田浦 保穂: "潜在性乳房炎と乳量・乳質および血清ビタミンA,Eの関連" 鹿児島大学農学部学術報告. 43. 49-57 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柳沢 知己,元村 泰彦 上村 俊一,浜名 克己: "泌乳牛血液由来の好中球Nitroblue Tetrazo-rium(NBT)還元能に及ぼすRetinolとα-Tocopherclの効果" 日本獣医師会雑誌. 46,5. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 元村 泰彦,柚木 弘之 上村 俊一,浜名 克己: "乳牛の乳頭測定値と乳質、細菌検査の関連" 日本獣医師会雑誌. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 元村 泰彦,安田 宣紘 上村 俊一,浜名 克己: "乳牛の正常および異常乳頭の超音波診断と形態学的観察" 日本獣医師会雑誌. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hamana, K., Kudo, Y. and Taura, Y.: "Effects of subclinical mastis on milk components and serum vitamin A and E." The Bulletin of the Faculty of Agriculture, Kagoshima University. 43. 49-57 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagisawa, T., Motomura, Y., Kamimura, S. and Hamana, K.: "Effect of retinol and alphatocopherol on nitroblue tetrazolium (NBT) reduction ability of neutrophils from blood of lactating cows." J. Jpn. Vet. Med. Assoc.46. 5 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motomura, Y., Yugi, H., Kamimura, S. and Hamana, K.: "Teat morphology related to milk quality and bacteria in dairy cattle." J. Jpn. Vet. Med. Assoc.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motomura, Y., Yasuda, N., Kamimura, S. and Hamana, K.: "Ultrasonic tomography and morphological observation on normal and abnormal teat of dairy cattle." J. Jpn. Vet. Med. Assoc.(1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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