1992 Fiscal Year Final Research Report Summary
Activation Mechanism of Hepatocyte Growth Factor Sequestered in Extracellular matrix in liver
| Project/Area Number |
03670016
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kitasato University |
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Principal Investigator |
YAMAMOTO Noboru School of Nursing, Kirasato Univ.Professor, 看護学部, 教授 (10050543)
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| Co-Investigator(Kenkyū-buntansha) |
MASUMOTO Aki Kitasato Univ.Assistance, 看護学部, 助手 (30209457)
MATSUTANI Sinji Kitasato Univ.Assistance, 看護学部, 助手 (60219433)
HASEGAWA Takayuki Kitasato Univ.Assistance Professor, 看護学部, 講師 (60180858)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Latent HGF / latent HGF-activator / Activator of pro-HGF activator / liver injury / Extracellular Matrix |
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| Research Abstract |
We have demonstrated that hepatocyte growth factor (HGF) is secreted from nonparenchymal liver cells and that a large amount of HGF is sequestered in the extracellular matrix(ECM) in Disse's space in the normal adult rat liver.
We also observed in this study that substances secreted from the primary cultured hepatocytes could release HGF from HGF-bound ECM.Analysis of the activating mechanism of latent HGF sequestered in ECM shows that two different substances, a proactivator of latent HGF (proactHGF) and an activating factor of proactHGF, participate in the activating latent HGF.A large amount of proactHGF is sequestered in ECM in normal rat liver.
These results indicate th epossibility that a cascade mechanism, first release an activating factor of proactHGF from hepatocytes after liver injury folowing activation(release) of latent HGF sequestered in ECM between the layr of hepatocyes and enthothelial cells forming sinusoid.
Thus, we postulate that a physiologically relevant protease, an activating factor of proactHGF, secreted from the partially injured hepatocytes has a key in the reparing mechanism after liver injury.
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