• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Subcellular Regulation of Calcium Channel Currents in Renal Tubules.

Research Project

Project/Area Number 03670036
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionChiba Univ. School of Medicine (1992)
The University of Tokyo (1991)

Principal Investigator

KAWAHARA Katsumasa  Chiba U. School of Med., Physiol., Instructor, 医学部, 講師 (70134525)

Project Period (FY) 1991 – 1992
KeywordsRenal Tubule / Patch-Clamp / Fluorescence Microscopy / Extracellular ATP / Intracellular Ca / G-protein / Cation Channel / Mn Permeable
Research Abstract

I have used the patch-clamp technique to identify and study Ca permeable channels in cultured renal distal tubule cells (A6 cells). The pipette solutions contained (in mM) Nagluconate 100, NaCl 20, MgCl_2 1, NaHEPES (pH 7.4) 10, EGTA 0.1-5. The bath either contained a normal amphibian solution or a Ca free solution. In the whole-cell clamp recording, the inward cation currents were induced by application of ATP (0.2-20 uM) to the cell at the equilibrium potential of Cl (E_<cl>=-47 mV). The currents were almost completely abolished when the pipette contained 5 mM EGTA and the cells were placed in a Ca free solution. Pretreatment of the cells with pertussis toxin (1 ug/ml)slowed the activation of the inward currents in the absence but not in the presence of extracellular Ca. When NaCl was totally replaced by CaCl_2 (45) or CaCl_2 (22.5) + MnCl_2 (22.5)(in mM), the Ca and/or Mn currents were evoked by ATP at E_<cl> (-37 mV). Conclusion: The external ATP opens the Ca and Mn permeable cation channel which is sensitive to the intracellular Ca activity.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] KAWAHARA,K.& MATSUZAKI,K.: "Activation of calcium channel by shear-stress in cultured renal tubule cells." Biochem.Biophys.Res.Commun.184. 198-205 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "非興奮性膜のNa^+チャネル" Clinical Neuroscience. 10. 401-404 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "カリウム チャネル" 腎と透析. 33. 447-452 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "細胞容積調節におけるイオンチャネルの役割" Clinc Neuroscience. 11. 223 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "腎疾患モデル" 東京医学社, 624 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "Annual Review 腎臓 1993" 中外医学社, 249 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KAWAHARA, K. and MATSUZAKI, K.: "ACTIVATION OF CALCIUM CHANNEL BY SHEAR-STRESS IN CULTURED RENAL DISTAL TUBULE CELLS." Biochem. Biophys. Res. Commun.184. 198-205 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KAWAHARA,K. and TAKUWA, N.: "BOMBESIN ACTIVATES LARGE-CONDUCTANCE CHLORIDE CHANNELS IN SWISS 3T3 FIBROBLASTS." Biochem. Biophys. Res. Commun.177. 292-298 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi