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1992 Fiscal Year Final Research Report Summary

Optical recording of the synaptic functions of the frog lumbar sympathetic ganglia using voltage-sensitive dyes.

Research Project

Project/Area Number 03670051
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SAKAI Tetsuro  Tokyo Medical and Dental University, School of Medicine, Lecturer., 医学部, 講師 (40153845)

Co-Investigator(Kenkyū-buntansha) ADANIYA Hitoshi  Tokyo Medical and Dental University, School of Medicine, Instructor., 医学部, 助手 (20202640)
Project Period (FY) 1991 – 1992
Keywordsvoltage-sensitive dye / optical recording / sympathetic ganglion / synapse
Research Abstract

In the preparations of the lumbar sympathetic ganglion dissected from bullfrogs, the neural responses to electrical stimulation were monitored by using multiple-site optical recording of electrical activity,with a voltage- sensitive merocyanine-rhodanine dye (NK2761) and a multiple element photodiode array. Voltage-related absorbance changes evoked by nerve stimulation with depolarizing (positive) square current pulses using a suction electrode were recorded simultaneously from many contiguous loci in the preparation.
The optical signals, evoked by the antidromic stimulation for the post- synaptic axons of the ganglion cells (post-stimulation), exhibited a relatively simple shape with a sharp spike followed by the undershoot, which is likely to reflect the action potential and the after-hyperpolarization of the ganglion cells respectively. On the other hand, the optical signals, evoked by orthodromic stimulation applied to the pre-synaptic fibers (pre-stimulation), were also spike-like but more complex in shape, and the double-peaked signals were often observed, suggesting that the component from pre-synaptic neurons and that from post-synaptic neurons were superimposed in the evoked optical signals.
Using a low-Ca^<2+> Ringer solution or d-tubocurarine (d-Tc) to suppress the synaptic transmission, the optical signals evoked by pre-stimulation were successively separated into pre-synaptic and post-synaptic components. The pre- synaptic component of the optical signals was resistant to the suppression of the synaptic transmission, and was suggested to be dependent on the external Ca^<2+>.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] T.SAKAI: "Optical determination of impulse conduction velosity during development of embryonic chick cervical vagus nerve bundles." Journal of Physiology(London). 439. 361-381 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.KOMURO: "Optical detction of postsynaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem.slice." Journal of Physiology(London). 442. 631-648 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.MOMOSE-SATO: "Optical mapping the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology(London). 442. 649-668 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.MOMOSE-SATO: "Optical monitoring of cholinergic postsynaptic potential in the embryonic chick ciliary ganglion using a voltage-sensitive dye." Biomedical Research. 12supp1.2. 139-140 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.KOMURO: "Optical monitoring of early appearance of spontaneous membrane potential changes in the embryonic chick medulla oblongata using a voltage-sensitive dy" Neuroscience.52. 55-62 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.SAKAI: "Optical mapping of neural response patterns to glosso-pharyngeal nerve stimulation in the embryonic chick brain stem." Society for Neuroscience Abstracts.18. 946 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.KAMINO: "Fluorscence Spectroscopy:New Methods and Applications" Springer-Verlag,Berlin,Germany Ed。by O.S.Wolfbeis, 310 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai, T., Komuro, H., Katoh, Y., Sasaki, H., Momose-Sato, Y. & Kamino, K.: "Optical determination of impulse conduction velocity during development of embryonic chick cervical vagus nerve bundles." Journal of Physiology (London). 439. 361-381 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komuro, H., Sakai, T., Momose-Sato, Y., Hirota, A. & Kamino, K.: "Optical detection of postsynaptic potentials evoked by vagal stimulation in the early embryonic chick brain stem slice." Journal of Physiology (London). 442. 631-648 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Momose-Sato, Y., Sakai, T., Komuro, H., Hirota, A. & Kamino, K.: "Optical mapping of the early development of the response pattern to vagal stimulation in embryonic chick brain stem." Journal of Physiology (London). 442. 649-668 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Momose-Sato, Y., Komuro, H., Sakai, T., Hirota, A. & Kamino, K.: "Optical monitoring of cholinergic postsynaptic potential in the embryonic chick ciliary ganglion using a voltage-sensitive dye." Biomedical Research 12 Supplement. 2. 139-140 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamino, K., Sakai, T., Momose-Sato, Y., Komuro, H., Hirota, A. & Sato, K.: "Optical monitoring of postsynaptic potential in the early embryonic avian brain stem using a voltage-sensitive dye." Fluorescence Spectroscopy: New Methods and Applications. ed. O.S. Wolfbeis, Springer-Verlag, Berlin, Germany.289-301 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komuro, H., Momose-Sato, Y., Sakai, T., Hirota, A. & Kamino, K.: "Optical monitoring of early appearance of spontaneous membrane potential changes in the embryonic chick medulla oblongata using a voltage-sensitive dye." Neuroscience. 52. 55-62 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamino, K., Sakai, T., Momose-Sato, Y., Komuro, H., Hirota, A. & Sato, K.: "Optical indications of early appearance of postsynaptic potentials in the early embryonic chick brain stem." Japanese Journal of Physiology. 43 Supplement. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Momose-Sato, Y., Sakai, T., Hirota, A., Sato, K. & Kamino, K.: "Optical monitoring of glutaminergic excitatory postsynaptic potentials from the early developing embryonic chick brain stem slice." Annals of New York Academy of Sciences,. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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