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1993 Fiscal Year Final Research Report Summary

MOLECULAR AND CELLULAR MECHANISM UNDERLYING NEURONAL REGENERATION IN HYPOTHALAMUS

Research Project

Project/Area Number 03670073
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 環境生理学(含体力医学・栄養生理学)
Research InstitutionGUNMA UNIVERSITY

Principal Investigator

ISHIKAWA Koichi  GUNMA UNIVERSITY,ASSOCIATE PROFESSOR, 生体調節研究所, 助教授 (80143238)

Co-Investigator(Kenkyū-buntansha) OHE Yoshihide  GUNMA UNIVERSITY,ASSISTANT PROFESSOR, 生体調節研究所, 教務員 (80125830)
Project Period (FY) 1991 – 1993
Keywordspia mater / arachnoid / regeneration / vasopressin / IGF-II / IGFBP-2 / beta-trace protein
Research Abstract

To clarify the cellular mechanisms through which hypothalamic magnocellular neurons send their fibers to the terminal region, namely the pituitary including the median eminence, we have performed several types of experiments. First, we have found that the median eminence was composed with the POMC-containing neuronal cell bodies, in addition to neuronal fibers, blood vessel-devived cells and pia mater-derived cells(Neuroendocrinology, 1992). During the developmental and/or regeneration process, only pia mater cells changed coordinately with the fiber elongation, suggesting the importance of the pia mater in the regeneration process. Further, the pia mater tissues implanted onto the surface of the median eminence were heavily invaded with regene-rating fibers after hypophysectomy(BRAIN RES,in press). These finding may indicate the existing of an important factor(s) releasable from the pia mater cells during developmental and/or regeneration process. In the next step, we prepared meninge … More al(the pia mater and arachnoid)cell cultures and then collected the medium conditioned by the cultured cells. The medium was found to contain a certain neurotrophic factor(s) using an in vitro system. The factor(s) was differed from FGF in the action spectrum on the neuronal cell survival(Neuroendocrinology, 1992). Finally, we have found that the neurotrophic activity is due to multiple factors contained in the medium, but mainly through IGF-II and IGFBP-2(Sinkeikagaku, in Japanese, 1994).In addition to IGF-II/IGFBP-2, the medium contained extremely low levels of an unidentified factor which was essential for IGF-II actions on neuronal cells. Further more, the medium was found to contain a lot of protein molecules which were very important in the regulation of neuronal survival or maintenance. For example, beta-trace protein was shown to be secreted from the pia mater cells. The preset studies raise the possibility, therefore, that the pia mater(and/or arachnoid)plays important roles in the regulation not only the blood/CSF barrier but also the parenchymal cell functions in the brain. Less

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Ishikawa,K.: "Pro-opiocortin-containing neurons in rat median eminence." Neuroendocrinology. 56. 178-184 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa,K.: "Neurotrophic effects of fibroblast growth factors on peptide-containing neurons in culture from postnatal rat hypothalamus" Neuroendocrinology. 55. 193-198 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinoda,M.: "Morphological changes of somatostatin neurons in the suprachiasmatic nucleus by hypophysectomy of the rat." Exp.Anim.43. 333-341 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa,K.: "Meninges play a neurotrophic role in the regeneration of vasopressin nerves after hypophysectomy" Brain Research,in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石川巧一: "培養脳軟膜細胞は IGF-II/IGFBP-2とβ-trace protsinを産生する" 神経化学. 33. 456-457 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa, K., Katakai, K., Tanaka, S., Haga, S.Mochida, H., and Itoh, K.: "Pro-opiomelanocortin-containing neurons in rat median eminence." Neuroendocrinology. 56. 178-184 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, K., Ohe, Y., Okutomi, Y., Takeuchi, T.and Suzuki, M.: "Neurotrophic effects of fibroblast growth factors on peptide-containing neurons in culture from postnatal rat hypothalamus." Neuroendocrinology. 55. 193-198 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinoda, M., Ishikawa, K., and Miura, T.: "Morphological changes of somatostatin neurons in the suprachiasmatic nucleus by hypophysectomy of the rat." Exp.Anim.43(3). 333-341 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, K., Kabeya, K., Shinoda, M., Katakai, K., Mori, M., and Tatemoto, K.: "Meninges play a neurotrophic role in the regeneration of vasopressin nerves after hypophysectomy." Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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