1992 Fiscal Year Final Research Report Summary
Change of GTP-binding Protein in Hormone Producing Tumors of Rats
| Project/Area Number |
03670118
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Inst. Basic Med. Sci., University of Tsukuba |
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Principal Investigator |
MIWA Masanao Inst. Basic Med. Sci., Professor, 基礎医学系, 教授 (20012750)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA K Inst. Basic Med. Sci., Assist. Prof, 基礎医学系, 講師 (90211078)
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| Project Period (FY) |
1991 – 1992
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| Keywords | G-protein / ADP-ribosylation / Hormone producing tumor / Ovarian tumor / mutation / Gs protein / Gi protein / signal transduction |
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| Research Abstract |
We have been working on the function of ADP-ribosylation reaction in various cellular mechanisms including signal transduction and cell growth.
ADP-ribosylation of GTP-binding proteins play important roles in cellular signal transduction, in particular, activation of adenylate cyclase system.
Recent finding of mutation at the site of ADP-ribosylation of Gs and Gi proteins in hormone producing tumors of human by Bourne's group made us to investigate the similar changes in various tumors including experimental rat tumors of pancreas. Although we could not find the mutation in Gs proteins in various rat tumors but could find the mutation in one of the ten hormone producing human ovarian tumors. This is the first finding of the mutation of alpha subunit of Gs protein in human ovarian tumors. We could not find the mutation of Gi protein in our cases with hormone producing human ovarian tumors. We are currently identifying the exact site of mutation in the mutated Gsprotein in the ovarian tumor.
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[Publications] Armato,U., Testolin,L., Menegazzi, M., Menapace, L., Ribecco, M., Carcereri de Prati, A., Miwa, M. and Suzuki, H.: "The exposure of carcinogen-initiated primary neonatal rat hepatocytes to tumor promoters modulates both the transcripts and the enzymatic activity of nuclear poly(ADP-ribose) polymerase." Biochem.Biophys.Res.Commun.182. 1066-1074 (1992)
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[Publications] Menegazzi, M., Suzuki, H., Carcereri de Prati, A., Tommasi,M., Miwa, M., Candini, G. and Cerosa, F.: "Increase of poly(ADP-ribose) polymerase mRNA levels during TPA-induced differentiation of human lymphocytes." FEBS Lett. 297. 59-62 (1992)
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[Publications] Cesarone, C.F., Suzuki, H., Scovassi, A.I., Scarabelli, L., Izzo, R., Giannoni, P., Mariani, C., Miwa, M., Orunesu, M. and Bertazzoni, U.: "Influence of poly(ADP-ribose) polymerase depletion on promotion of liver carcinogenesis." Molecular Carcinogenesis. 5. 111-117 (1992)
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[Publications] Miwa, M., Ozawa, Y., Uchida, M., Kushida, S., Ami, Y., Matsumuta, M. and Uchida, K.: "Cloning of poly(ADP-ribose) polymerase cDNA from lower eucaryotes" In: ADP-ribosylation Reactions (Poirier, G.G., Moreau, P. eds.) Sprnger-Verlag, New York,. 14-19 (1992)
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[Publications] Miwa, M., Uchida, M., Akiyama, T. and Uchida, K.: "Detection and analysis of NAD binding proteins including poly(ADP-ribose) polymerase immobilized on the membrane" In: ADP-ribosylation Reactions (Poirier, G.G., Moreau, P. eds.) Sprnger-Verlag, New York,. 125-128 (1992)
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[Publications] Uchida, K., Suzuki, H., Maruta, H., Abe, H., Aoki, K., Miwa, M. and Tanuma, S.: "Preferential degradation of protein-bound(ADP-ribose)n by nuclear poly(ADP-ribose) glycohydrolase from human placenta." J. Biol. Chem.268. 3194-3200 (1993)
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[Publications] Miwa, M. and Uchida, K.: In: The Post-translational Modification of Proteins-Roles in Molecular and Cellular Biology (Tsuboi, S., Katsunuma, N., Taniguchi, N. eds.) Japan Scientific Societies Press, Tokyo. Poly(ADP-ribose) polymerase: structure, expression, and biological function., 171-181 (1992)
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