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1992 Fiscal Year Final Research Report Summary

Molecular mechanism of transcriptional regulation by insulin

Research Project

Project/Area Number 03670122
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

NOGUCHI Tamio  Osaka University Nutr. & Physiol. Chem Assoc. Pro, 医学部, 助教授 (70135721)

Project Period (FY) 1991 – 1992
KeywordsL-type pyruvate kinase gene / Glucokinase gene / Insulin response element / Transgeninic mice
Research Abstract

We produced transgenic mice containing the 5'-flanking region of the L-type pyruvate kinase (LPK) gene from nucleotide -189 50 +37, which includes an enhancer unit and TATA box as functional elements, linked to the chloramphenicol acetyltransferase (CAT) gene. Transgene expression was stimulated by both dietary glucose and fructose in the liber. The fusion gene was also transfected into cultured hepatocytes and the cells were incubated with or without insulin. Insulin stimulated expression of the CAT gene in the presence of glucose, but not in the presence of pyruvate. Thus, we suggest that an enhancer unit of the LPK gene is insulin response unit.
2. HNF1 binds to L-III, which constitutes an enhancer unit of the LPK gene. Expression of HNF1 was not under dietary control.
3. Using CAT assay, we showed that the 5'-flanking region of the glucokinase up to -1100 contained the DNA region responsive to insulin.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Imai,Enyu: "Alteration in L-type pyruvate kinase bene expression is not associated with the LF-B1 mRNA level." Biochemical and Biophysical Research Communications. 176. 1074-1078 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuchi,Tamio: "Expression of liver type pyruvate kinase in insulinoma:involvement of LF-Bl." Biochemical and Biophysical Research Communications. 181. 259-264 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi,Tamio: "Molecular mechanism of induction of key enzymes related to lipogenesis." Proceedings of the Society for Experimantal Biology and Medicine. 200. 206-209 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi,Tamio: "An enhancer unit of L-ytpe pyruvate kinase gene is responsible for troancriptional stimulation by dietary fructose as well as glucose in tranenic mice" FEBS Letters. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imai, E. et al: "Alteration in L-type pyruvate kinase gene expression is not associated with the LH-B1 mRNA level" Biochem. Biophys. Res. Commun. 176. 1074-1078 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, T. et al: "Expression of liver type pyruvate kinase in insulinoma: involvement of LF-B1 (HNF1)" Biochem. Biophys. Res. Commun. 181. 259-264 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, T. et al: "Molecular mechanism of induction of key enzymes related to lipogenesis" Proc. Soc. Exp. Biol. Med. 200. 206-209 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, T. et al: "An enhancer unit of L-type pyruvate kinase gene is responsible for transcriptional stimulation by dietary fructose as well as glucose in transgenic mice" FEBS Lett. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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